The Spinal Muscular Atrophy Disease Protein SMN Is Linked to the Golgi Network

Chen Hung Ting, Hsin Lan Wen, Hui Chun Liu, Hsiu Mei Hsieh-Li, Hung Li, Sue Lin-Chao

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Proximal spinal muscular atrophy (SMA) is a neurodegenerative disorder caused by deficiency of the ubiquitous Survival of Motor Neuron (SMN) protein. SMN has been shown to be transported in granules along the axon and moved through cytoskeletal elements. However, the role and nature of SMN granules are still not well characterized. Here, using immunocytochemical methods and time-lapse studies we show that SMN granules colocalize with the Golgi apparatus in motor neuron-like NSC34 cells. Electron microscopy clearly revealed that SMN granules are transported into the Golgi stack and aggregate in the trans-Golgi apparatus. SMN granules are characterized as either coated or un-coated and behave like regulated secretory granules. Treatment of cells with monensin to disrupt Golgi-mediated granule secretion decreased SMN expression in neurites and caused growth cone defects similar to those seen in SMN knockdown cells. Knockdown of Cop-α, the protein that coats vesicles transporting proteins between the Golgi compartments, caused SMN granule accumulation in the Golgi apparatus. In addition to the well-studied role of SMN in small nuclear ribonucleoprotein (SnRNP) assembly, this work links SMN granules with the Golgi network and thus sheds light on Golgi-mediated SMN granule transport.

Original languageEnglish
Article numbere51826
JournalPloS one
Volume7
Issue number12
DOIs
Publication statusPublished - 2012 Dec 17

Fingerprint

Spinal Muscular Atrophy
muscular atrophy
Motor Neurons
Muscular Diseases
motor neurons
Neurons
granules
Proteins
proteins
Golgi Apparatus
Golgi apparatus
Small Nuclear Ribonucleoproteins
Monensin
ribonucleoproteins
Growth Cones
neurites
monensin
secretory granules
Secretory Vesicles
neurodegenerative diseases

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

The Spinal Muscular Atrophy Disease Protein SMN Is Linked to the Golgi Network. / Ting, Chen Hung; Wen, Hsin Lan; Liu, Hui Chun; Hsieh-Li, Hsiu Mei; Li, Hung; Lin-Chao, Sue.

In: PloS one, Vol. 7, No. 12, e51826, 17.12.2012.

Research output: Contribution to journalArticle

Ting, Chen Hung ; Wen, Hsin Lan ; Liu, Hui Chun ; Hsieh-Li, Hsiu Mei ; Li, Hung ; Lin-Chao, Sue. / The Spinal Muscular Atrophy Disease Protein SMN Is Linked to the Golgi Network. In: PloS one. 2012 ; Vol. 7, No. 12.
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