The solution structure of the active domain of CAP18 - a lipopolysaccharide binding protein from rabbit leukocytes

Chinpan Chen, Roland Brock, Frederick Luh, Ping Jung Chou, James W. Larrick, Rong Fong Huang, Tai huang Huang

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We have employed the circular dichroism (CD) technique to characterize the solution structure of CAP18106-137, a lipopolysaccharide (LPS) binding, antimicrobial protein, and its interaction with lipid A. Our results revealed that CAP18106-137 may exist in at least three lipid A concentration-dependent, primarily helix conformations. The 'model' structure of CAP18106-137 in 30% (v/v) TFE, determined by nuclear magnetic resonance (NMR) technique, was found to be a complete and very rigid helix. In this conformation, the cationic and hydrophobic groups of CAP18106-137 are separated into patches and stripes in such a way that it can favorably interact with lipid A through either coulombic interaction with the diphosphoryl groups or hydrophobic interaction with the fatty acyl chains.

Original languageEnglish
Pages (from-to)46-52
Number of pages7
JournalFEBS Letters
Volume370
Issue number1-2
DOIs
Publication statusPublished - 1995 Aug 14

Fingerprint

Lipid A
Leukocytes
Rabbits
Conformations
Polytetrafluoroethylene
Circular Dichroism
Model structures
Hydrophobic and Hydrophilic Interactions
Lipopolysaccharides
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
CAP18 lipopolysaccharide-binding protein
lipopolysaccharide-binding protein
Proteins

Keywords

  • Antibacterial peptide
  • Endotoxin
  • Lipid A
  • NMR

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The solution structure of the active domain of CAP18 - a lipopolysaccharide binding protein from rabbit leukocytes. / Chen, Chinpan; Brock, Roland; Luh, Frederick; Chou, Ping Jung; Larrick, James W.; Huang, Rong Fong; Huang, Tai huang.

In: FEBS Letters, Vol. 370, No. 1-2, 14.08.1995, p. 46-52.

Research output: Contribution to journalArticle

Chen, Chinpan ; Brock, Roland ; Luh, Frederick ; Chou, Ping Jung ; Larrick, James W. ; Huang, Rong Fong ; Huang, Tai huang. / The solution structure of the active domain of CAP18 - a lipopolysaccharide binding protein from rabbit leukocytes. In: FEBS Letters. 1995 ; Vol. 370, No. 1-2. pp. 46-52.
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