The secondary structure of a pyrimidine-guanine sequence-specific ribonuclease possessing cytotoxic activity from the oocytes of Rana catesbeiana

Chinpan Chen, Kellie Hom, Rong Fong Huang, Ping Jung Chou, You Di Liao, Tai-huang Huang

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Abstract

RC-RNase is a pyrimidine-guanine sequence-specific ribonuclease and a sialic-acid-binding lectin purified from Rana catesbeiana (bullfrog) oocytes. This 111-amino acid protein exhibits cytotoxicity toward several tumor cell lines. In this paper we report the assignments of proton NMR resonances and the identification of the secondary structure deduced from NOE constraints, chemical shift index, 3JNHα and amide proton exchange rates. The protein was directly isolated from bullfrog oocytes; we were able to assign all but five of the amino acid backbone protons of the unlabeled protein by analyzing a large set of two-dimensional proton NMR spectra obtained at several temperatures and pH conditions. Our results indicate that the structure of RC-RNase is dominated by the presence of two triple-stranded anti-parallel β-sheets and three α-helices, similar to those of the pyrimidine family ribonucleases. Two sets of resonances were observed for 11 amide protons and 8 α-protons located in the loop-1 region, an α2 helix, and three β-strands (β1, β3 and β4), suggesting the presence of nonlocalized multiple conformations for RC-RNase.

Original languageEnglish
Pages (from-to)331-344
Number of pages14
JournalJournal of Biomolecular NMR
Volume8
Issue number3
DOIs
Publication statusPublished - 1996 Jan 1

Fingerprint

Rana catesbeiana
Guanine
Ribonucleases
Oocytes
Protons
Amides
Nuclear magnetic resonance
Amino Acids
Proteins
Chemical shift
N-Acetylneuraminic Acid
Cytotoxicity
Tumor Cell Line
Lectins
Conformations
pyrimidine
Tumors
Cells
Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

Cite this

The secondary structure of a pyrimidine-guanine sequence-specific ribonuclease possessing cytotoxic activity from the oocytes of Rana catesbeiana. / Chen, Chinpan; Hom, Kellie; Huang, Rong Fong; Chou, Ping Jung; Liao, You Di; Huang, Tai-huang.

In: Journal of Biomolecular NMR, Vol. 8, No. 3, 01.01.1996, p. 331-344.

Research output: Contribution to journalArticle

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