The recycling of L-citrulline to L-arginine in a chicken macrophage cell line

Chun-Li Su, R. E. Austic

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

L-Arginine is the only biological substrate of nitric oxide synthase in a reaction yielding NO and L-citrulline as co-products. The resynthesis of L-arginine from L-citrulline has been observed in murine macrophages. However, it is not known whether avian macrophages have a similar capacity for the synthesis of arginine. The present studies were carried out to determine whether L-citrulline can support NO (measured as nitrite) production in the HD11 cell, a chicken macrophage cell line. When added to media lacking L-arginine, L-citrulline supported a low level of nitrite accumulation: about 4 to 11% of the amount of nitrite formed from an equivalent concentration of L-arginine. Aspartic acid was not limiting for NO production from citrulline.

Original languageEnglish
Pages (from-to)353-355
Number of pages3
JournalPoultry Science
Volume78
Issue number3
DOIs
Publication statusPublished - 1999 Jan 1

Fingerprint

citrulline
recycling
arginine
macrophages
cell lines
chickens
nitrites
coproducts
aspartic acid
nitric oxide synthase
synthesis
mice
cells

Keywords

  • Aspartic acid
  • Citrulline
  • HD11 cell
  • Nitric oxide
  • Nitrite

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

The recycling of L-citrulline to L-arginine in a chicken macrophage cell line. / Su, Chun-Li; Austic, R. E.

In: Poultry Science, Vol. 78, No. 3, 01.01.1999, p. 353-355.

Research output: Contribution to journalArticle

@article{0f596522b8a84ba1abb01ffca85f68b5,
title = "The recycling of L-citrulline to L-arginine in a chicken macrophage cell line",
abstract = "L-Arginine is the only biological substrate of nitric oxide synthase in a reaction yielding NO and L-citrulline as co-products. The resynthesis of L-arginine from L-citrulline has been observed in murine macrophages. However, it is not known whether avian macrophages have a similar capacity for the synthesis of arginine. The present studies were carried out to determine whether L-citrulline can support NO (measured as nitrite) production in the HD11 cell, a chicken macrophage cell line. When added to media lacking L-arginine, L-citrulline supported a low level of nitrite accumulation: about 4 to 11{\%} of the amount of nitrite formed from an equivalent concentration of L-arginine. Aspartic acid was not limiting for NO production from citrulline.",
keywords = "Aspartic acid, Citrulline, HD11 cell, Nitric oxide, Nitrite",
author = "Chun-Li Su and Austic, {R. E.}",
year = "1999",
month = "1",
day = "1",
doi = "10.1093/ps/78.3.353",
language = "English",
volume = "78",
pages = "353--355",
journal = "Poultry Science",
issn = "0032-5791",
publisher = "Poultry Science Association",
number = "3",

}

TY - JOUR

T1 - The recycling of L-citrulline to L-arginine in a chicken macrophage cell line

AU - Su, Chun-Li

AU - Austic, R. E.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - L-Arginine is the only biological substrate of nitric oxide synthase in a reaction yielding NO and L-citrulline as co-products. The resynthesis of L-arginine from L-citrulline has been observed in murine macrophages. However, it is not known whether avian macrophages have a similar capacity for the synthesis of arginine. The present studies were carried out to determine whether L-citrulline can support NO (measured as nitrite) production in the HD11 cell, a chicken macrophage cell line. When added to media lacking L-arginine, L-citrulline supported a low level of nitrite accumulation: about 4 to 11% of the amount of nitrite formed from an equivalent concentration of L-arginine. Aspartic acid was not limiting for NO production from citrulline.

AB - L-Arginine is the only biological substrate of nitric oxide synthase in a reaction yielding NO and L-citrulline as co-products. The resynthesis of L-arginine from L-citrulline has been observed in murine macrophages. However, it is not known whether avian macrophages have a similar capacity for the synthesis of arginine. The present studies were carried out to determine whether L-citrulline can support NO (measured as nitrite) production in the HD11 cell, a chicken macrophage cell line. When added to media lacking L-arginine, L-citrulline supported a low level of nitrite accumulation: about 4 to 11% of the amount of nitrite formed from an equivalent concentration of L-arginine. Aspartic acid was not limiting for NO production from citrulline.

KW - Aspartic acid

KW - Citrulline

KW - HD11 cell

KW - Nitric oxide

KW - Nitrite

UR - http://www.scopus.com/inward/record.url?scp=0033091021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033091021&partnerID=8YFLogxK

U2 - 10.1093/ps/78.3.353

DO - 10.1093/ps/78.3.353

M3 - Article

C2 - 10090261

AN - SCOPUS:0033091021

VL - 78

SP - 353

EP - 355

JO - Poultry Science

JF - Poultry Science

SN - 0032-5791

IS - 3

ER -