The possible interaction of CDA14 and protein elongation factor 1α

Ying Fang Yang, Min Yuan Chou, Chia Yu Fan, Sung-Fang Chen, Ping Chiang Lyu, Chung Cheng Liu, Tzu Ling Tseng

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The CDA14, a 45kD protein, is currently annotated as PTX1-like protein or ERGIC 32. Over expressing CDA14 can slow PC11 cell proliferation rate. In HepG2 cells, it had been demonstrated that CDA14 is involved in protein transportation. The knowledge about the protein is very limited and not clarified. The CDA14 and its homologous proteins form a family and are restricted to eukaryotes. In the family, there are no homologous sequences with resolved three-dimensional structure and their functions are difficult to predict. Transcriptional expression of CDA14 in three hepatoma cell lines, Hu7, HCC and HepG2, was lower than normal liver tissue and liver carcinoma tissue. In this study, functional proteomic techniques were utilized in searching the interacting counterpart of CDA14. Several proteins involved in protein translation and folding were selectively precipitated with CDA14 and identified mass spectrometry. Interaction of CDA14 and elongation factor 1α was confirmed by Western blotting and confocal microscopy. Elongation factor 1α is a multiple function protein and involved in several biological mechanisms, including protein synthesis, cell proliferation, apoptosis and tumorigensis. Over-expression of CDA14 down regulated the proliferation of HepG2 cells. These results suggest that CDA14 participated in the elongation factor 1α regulated mechanisms.

Original languageEnglish
Pages (from-to)312-318
Number of pages7
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1784
Issue number2
DOIs
Publication statusPublished - 2008 Feb 1

Fingerprint

Peptide Elongation Factor 1
Proteins
Hep G2 Cells
Cell proliferation
Liver
Cell Proliferation
Tissue
Protein Folding
Protein Biosynthesis
Sequence Homology
Confocal microscopy
Eukaryota
Confocal Microscopy
Proteomics
Hepatocellular Carcinoma
Mass Spectrometry
Mass spectrometry
Western Blotting
Apoptosis
Carcinoma

Keywords

  • CDA14
  • Cancer
  • Elongation factor 1α
  • Functional proteomic
  • HepG2
  • Single chain antibody

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics

Cite this

The possible interaction of CDA14 and protein elongation factor 1α. / Yang, Ying Fang; Chou, Min Yuan; Fan, Chia Yu; Chen, Sung-Fang; Lyu, Ping Chiang; Liu, Chung Cheng; Tseng, Tzu Ling.

In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1784, No. 2, 01.02.2008, p. 312-318.

Research output: Contribution to journalArticle

Yang, Ying Fang ; Chou, Min Yuan ; Fan, Chia Yu ; Chen, Sung-Fang ; Lyu, Ping Chiang ; Liu, Chung Cheng ; Tseng, Tzu Ling. / The possible interaction of CDA14 and protein elongation factor 1α. In: Biochimica et Biophysica Acta - Proteins and Proteomics. 2008 ; Vol. 1784, No. 2. pp. 312-318.
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