@article{d314d8a491114d3b96f83c0979d89faf,
title = "The N-terminus of TDP-43 promotes its oligomerization and enhances DNA binding affinity",
abstract = "TDP-43 is a DNA/RNA-binding protein associated with different neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD-U). Here, the structural and physical properties of the N-terminus on TDP-43 have been carefully characterized through a combination of nuclear magnetic resonance (NMR), circular dichroism (CD) and fluorescence anisotropy studies. We demonstrate for the first time the importance of the N-terminus in promoting TDP-43 oligomerization and enhancing its DNA-binding affinity. An unidentified structural domain in the N-terminus is also disclosed. Our findings provide insights into the N-terminal domain function of TDP-43.",
keywords = "DNA/RNA binding, N-terminus, Neurodegenerative disease, Oligomerization, TDP-43",
author = "Chang, \{Chung ke\} and Wu, \{Tzong Huah\} and Wu, \{Chu Ya\} and Chiang, \{Ming hui\} and Toh, \{Elsie Khai Woon\} and Hsu, \{Yin Chih\} and Lin, \{Ku Feng\} and Liao, \{Yu heng\} and Huang, \{Tai huang\} and Huang, \{Joseph Jen Tse\}",
note = "Funding Information: This work was supported by research grants from Academia Sinica and The National Science Council of the Republic of China (Grants NSC 97-2113-M-001-003-MY2, 98-2113-M-001-015-MY2, and NSC 100-2113-M-001-013-MY2 to J.J.H.; NSC100-2321-B-001-028 and NSC100-2311-B-001-023 to T.H.H.). T.-H.Y. was supported by Academia Sinica. We would like to thank Dr. Benjamin Tu and Dr. Hanna Yuan from Academia Sinica for providing full-length TDP-43, TDP 101–191 , TDP 192–265 and TDP 101–265 plasmids. We also thank the mass spectrometry support from Dr. Mei-Chun Tseng and the suggestions of NMR experiments from Dr. Chi-fon Chang and Sunney I. Chan. NMR experiments were carried out with NMR spectrometers of the High-Field Nuclear Magnetic Resonance Center (HFNMRC) supported by National Research Program for Genomic Medicine, The National Science Council of the Republic of China. ",
year = "2012",
month = aug,
day = "24",
doi = "10.1016/j.bbrc.2012.07.071",
language = "English",
volume = "425",
pages = "219--224",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier BV",
number = "2",
}