The Hippo pathway controls polar cell fate through Notch signaling during Drosophila oogenesis

Hsi Ju Chen, Chi Ming Wang, Tsu Wei Wang, Gwo Jen Liaw, Ta Hsing Hsu, Tzu Huai Lin, Jenn Yah Yu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

During Drosophila oogenesis, the somatic follicle cells form an epithelial layer surrounding the germline cells to form egg chambers. In this process, follicle cell precursors are specified into polar cells, stalk cells, and main-body follicle cells. Proper specification of these three cell types ensures correct egg chamber formation and polarization of the anterior-posterior axis of the germline cells. Multiple signaling cascades coordinate to control the follicle cell fate determination, including Notch, JAK/STAT, and Hedgehog signaling pathways. Here, we show that the Hippo pathway also participates in polar cell specification. Over-activation of yorkie (yki) leads to egg chamber fusion, possibly through attenuation of polar cell specification. Loss-of-function experiments using RNAi knockdown or generation of mutant clones by mitotic recombination demonstrates that reduction of yki expression promotes polar cell formation in a cell-autonomous manner. Consistently, polar cells mutant for hippo (hpo) or warts (wts) are not properly specified, leading to egg chamber fusion. Furthermore, Notch activity is increased in yki mutant cells and reduction of Notch activity suppresses polar cell formation in yki mutant clones. These results demonstrate that yki represses polar cell fate through Notch signaling. Collectively, our data reveal that the Hippo pathway controls polar cell specification. Through repressing Notch activity, Yki serves as a key repressor in specifying polar cells during Drosophila oogenesis.

Original languageEnglish
Pages (from-to)370-379
Number of pages10
JournalDevelopmental Biology
Volume357
Issue number2
DOIs
Publication statusPublished - 2011 Sep 15

Fingerprint

Polar Bodies
Oogenesis
Drosophila
Ovum
Clone Cells
Warts
Hedgehogs
RNA Interference
Genetic Recombination
Epithelial Cells

Keywords

  • Drosophila
  • Hippo pathway
  • Notch
  • Oogenesis
  • Polar cell

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

The Hippo pathway controls polar cell fate through Notch signaling during Drosophila oogenesis. / Chen, Hsi Ju; Wang, Chi Ming; Wang, Tsu Wei; Liaw, Gwo Jen; Hsu, Ta Hsing; Lin, Tzu Huai; Yu, Jenn Yah.

In: Developmental Biology, Vol. 357, No. 2, 15.09.2011, p. 370-379.

Research output: Contribution to journalArticle

Chen, Hsi Ju ; Wang, Chi Ming ; Wang, Tsu Wei ; Liaw, Gwo Jen ; Hsu, Ta Hsing ; Lin, Tzu Huai ; Yu, Jenn Yah. / The Hippo pathway controls polar cell fate through Notch signaling during Drosophila oogenesis. In: Developmental Biology. 2011 ; Vol. 357, No. 2. pp. 370-379.
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