The hemocompatibility of oxidized diamond nanocrystals for biomedical applications

Hung Cheng Li, Feng Jen Hsieh, Ching Pin Chen, Ming Yao Chang, Patrick C.H. Hsieh, Chia Chun Chen, Shain Un Hung, Che Chih Wu, Huan Cheng Chang

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26 Citations (Scopus)

Abstract

Low-dimensional carbon-based nanomaterials have recently received enormous attention for biomedical applications. However, increasing evidence indicates that they are cytotoxic and can cause inflammatory responses in the body. Here, we show that monocrystalline nanodiamonds (NDs) synthesized by high-pressure-high-temperature (HPHT) methods and purified by air oxidation and strong oxidative acid treatments have excellent hemocompatibility with negligible hemolytic and thrombogenic activities. Cell viability assays with human primary endothelial cells suggested that the oxidized HPHT-NDs (dimensions of 35-500 nm) are non-cytotoxic. No significant elevation of the inflammatory cytokine levels of IL-1β and IL-6 was detected in mice after intravenous injection of the nanocrystals in vivo. Using a hindlimb-ischemia mouse model, we demonstrated that 35-nm NDs after covalent conjugation with polyarginine are useful as a drug delivery vehicle of heparin for prolonged anticoagulation treatment. The present study lays a solid foundation for further therapeutic applications of NDs in biomedicine.

Original languageEnglish
Article number3044
JournalScientific Reports
Volume3
DOIs
Publication statusPublished - 2013 Oct 25
Externally publishedYes

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Cite this

Li, H. C., Hsieh, F. J., Chen, C. P., Chang, M. Y., Hsieh, P. C. H., Chen, C. C., Hung, S. U., Wu, C. C., & Chang, H. C. (2013). The hemocompatibility of oxidized diamond nanocrystals for biomedical applications. Scientific Reports, 3, [3044]. https://doi.org/10.1038/srep03044