The effect of hydration status on plasma fgf21 concentrations in humans: A subanalysis of a randomised crossover trial

Harriet A. Carroll*, Yung Chih Chen, Iain Templeman, Lewis J. James, James A. Betts, William V. Trim

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Aim Fibroblast growth factor 21 (FGF21) has recently been implicated in thirst in rodent models. The mechanisms for this are currently uncertain, and it is unclear whether hydration status can alter FGF21 concentrations, potentially providing an additional mechanism by which hypohydration induces thirst. The aim of this study is therefore to understand whether hydration status can alter circulating FGF21 in humans. Methods Using a heat tent and fluid restriction, we induced hypohydration (1.9% body mass loss) in 16 healthy participants (n = 8 men), and compared their glycaemic regulation to a rehydration protocol (heat tent and fluid replacement) in a randomised crossover design. Results After the hypohydration procedure, urine specific gravity, urine and serum osmolality, and plasma copeptin (as a marker for arginine vasopressin) increased as expected, with no change after the rehydration protocol. In the fasted state, the median paired difference in plasma FGF21 concentrations from the rehydrated to hypohydrated trial arm was -37 (interquartile range -125, 10) pg.mL-1(P = 0.278), with average concentrations being 458 ± 462 pg.mL-1 after hypohydration and 467 ± 438 pg.mL-1 after rehydration; mean difference -9 ± 173 pg.mL-1. Conclusion To our knowledge, these are the first causal data in humans investigating hydration and FGF21, demonstrating that an acute bout of hypohydration does not impact fasted plasma FGF21 concentrations. These data may suggest that whilst previous research has found FGF21 administration can induce thirst and drinking behaviours, a physiological state implicated in increased thirst (hypohydration) does not appear to impact plasma FGF21 concentrations in humans.

Original languageEnglish
Article numbere0235557
JournalPloS one
Issue number8 August 2020
Publication statusPublished - 2020 Aug

ASJC Scopus subject areas

  • General


Dive into the research topics of 'The effect of hydration status on plasma fgf21 concentrations in humans: A subanalysis of a randomised crossover trial'. Together they form a unique fingerprint.

Cite this