The conformations of trimethoprim/E. coli dihydrofolate reductase complexes A 15N and 31P NMR study

Fu yung Huang, Qing Xian Yang, Tai-huang Huang, Leslie Gelbaum, Lee F. Kuyper

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have employed 15N and 31P NMR techniques to characterize the conformations of trimethoprim (TMP)/E. coli dihydrofolate reductase (DHFR) complexes in the presence and absence of NADPH and NADP+. A single conformation was observed for TMP/DHFR, NADP+/DHFR, NADPH/ DHFR and TMP/NADPH/DHFR complexes. In the ternary complex of TMP/NADP+/DHFR both the 15N and 31P spectra revealed the presence of two conformations. However, the conformations of TMP and NADP+ in the ternary complex may not be correlated, resulting in the possible existence of four conformations for the protein ternary complex.

Original languageEnglish
Pages (from-to)44-46
Number of pages3
JournalFEBS Letters
Volume283
Issue number1
DOIs
Publication statusPublished - 1991 May 20

Fingerprint

Tetrahydrofolate Dehydrogenase
Trimethoprim
NADP
Escherichia coli
Conformations
Nuclear magnetic resonance
Ternary Complex Factors

Keywords

  • Conformation
  • Dihydrofolate reductase
  • E. coli
  • NMR, N, P, NADP
  • Trimethoprim

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The conformations of trimethoprim/E. coli dihydrofolate reductase complexes A 15N and 31P NMR study. / Huang, Fu yung; Yang, Qing Xian; Huang, Tai-huang; Gelbaum, Leslie; Kuyper, Lee F.

In: FEBS Letters, Vol. 283, No. 1, 20.05.1991, p. 44-46.

Research output: Contribution to journalArticle

Huang, Fu yung ; Yang, Qing Xian ; Huang, Tai-huang ; Gelbaum, Leslie ; Kuyper, Lee F. / The conformations of trimethoprim/E. coli dihydrofolate reductase complexes A 15N and 31P NMR study. In: FEBS Letters. 1991 ; Vol. 283, No. 1. pp. 44-46.
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