The catalytic domain of human hepatitis delta virus RNA. A proton nuclear magnetic resonance study

Bao Shiang Lee; Huey Nan Wu; Tai Huang Huang

doi:10.1016/0014-5793(93)80138-K

The catalytic domain of human hepatitis delta virus RNA. A proton nuclear magnetic resonance study

Bao Shiang Lee, Huey Nan Wu, Tai Huang Huang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We have obtained and analyzed the 600 MHz proton NMR spectra of a 74-mer RNA derived from the catalytic domain of hepatitis delta virus genomic RNA (HDV RNA) to determine its secondary structure. Deconvolution of the NMR spectrum obtained at 32°C indicates that part of the 74-mer RNA molecule may exist in multiple conformations in equilibrium. The major conformer contains two A-U base pairs and 14 ± 2 G-C base pairs. It appears to contain no standard G-U base pairs. Our NMR melting study suggests that this conformer has at least two stem-loop regions. One of the regions has been identified to be a tetra-loop. We have assigned five imino proton resonances of the tetraloop stem. Our data is consistent with the pseudoknot model of Perrotta and Been.

Original language	English
Pages (from-to)	296-300
Number of pages	5
Journal	FEBS Letters
Volume	324
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(93)80138-K
Publication status	Published - 1993 Jun 21
Externally published	Yes

Keywords

Hepatitis delta virus
NMR
RNA: Structure
Ribozyme

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0014-5793(93)80138-K

Cite this

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title = "The catalytic domain of human hepatitis delta virus RNA. A proton nuclear magnetic resonance study",

abstract = "We have obtained and analyzed the 600 MHz proton NMR spectra of a 74-mer RNA derived from the catalytic domain of hepatitis delta virus genomic RNA (HDV RNA) to determine its secondary structure. Deconvolution of the NMR spectrum obtained at 32°C indicates that part of the 74-mer RNA molecule may exist in multiple conformations in equilibrium. The major conformer contains two A-U base pairs and 14 ± 2 G-C base pairs. It appears to contain no standard G-U base pairs. Our NMR melting study suggests that this conformer has at least two stem-loop regions. One of the regions has been identified to be a tetra-loop. We have assigned five imino proton resonances of the tetraloop stem. Our data is consistent with the pseudoknot model of Perrotta and Been.",

keywords = "Hepatitis delta virus, NMR, RNA: Structure, Ribozyme",

author = "Lee, {Bao Shiang} and Wu, {Huey Nan} and Huang, {Tai Huang}",

note = "Funding Information: Ackrlo~~lrtigen?erzWtse thank Prof. B.C. Wang for the gtft of T7 RNA polymerase. Ms. Y.C. Lai and H.W. Hwang for asststancew tth sample preparation, and Ms. L.H. Tseng for asststance with NMR experiments. This work was supported by a grant from Nattonal Sctence Council of the Republic of Chma.",

year = "1993",

month = jun,

day = "21",

doi = "10.1016/0014-5793(93)80138-K",

language = "English",

volume = "324",

pages = "296--300",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "3",

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TY - JOUR

T1 - The catalytic domain of human hepatitis delta virus RNA. A proton nuclear magnetic resonance study

AU - Lee, Bao Shiang

AU - Wu, Huey Nan

AU - Huang, Tai Huang

N1 - Funding Information: Ackrlo~~lrtigen?erzWtse thank Prof. B.C. Wang for the gtft of T7 RNA polymerase. Ms. Y.C. Lai and H.W. Hwang for asststancew tth sample preparation, and Ms. L.H. Tseng for asststance with NMR experiments. This work was supported by a grant from Nattonal Sctence Council of the Republic of Chma.

PY - 1993/6/21

Y1 - 1993/6/21

N2 - We have obtained and analyzed the 600 MHz proton NMR spectra of a 74-mer RNA derived from the catalytic domain of hepatitis delta virus genomic RNA (HDV RNA) to determine its secondary structure. Deconvolution of the NMR spectrum obtained at 32°C indicates that part of the 74-mer RNA molecule may exist in multiple conformations in equilibrium. The major conformer contains two A-U base pairs and 14 ± 2 G-C base pairs. It appears to contain no standard G-U base pairs. Our NMR melting study suggests that this conformer has at least two stem-loop regions. One of the regions has been identified to be a tetra-loop. We have assigned five imino proton resonances of the tetraloop stem. Our data is consistent with the pseudoknot model of Perrotta and Been.

AB - We have obtained and analyzed the 600 MHz proton NMR spectra of a 74-mer RNA derived from the catalytic domain of hepatitis delta virus genomic RNA (HDV RNA) to determine its secondary structure. Deconvolution of the NMR spectrum obtained at 32°C indicates that part of the 74-mer RNA molecule may exist in multiple conformations in equilibrium. The major conformer contains two A-U base pairs and 14 ± 2 G-C base pairs. It appears to contain no standard G-U base pairs. Our NMR melting study suggests that this conformer has at least two stem-loop regions. One of the regions has been identified to be a tetra-loop. We have assigned five imino proton resonances of the tetraloop stem. Our data is consistent with the pseudoknot model of Perrotta and Been.

KW - Hepatitis delta virus

KW - NMR

KW - RNA: Structure

KW - Ribozyme

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U2 - 10.1016/0014-5793(93)80138-K

DO - 10.1016/0014-5793(93)80138-K

M3 - Article

C2 - 8405369

AN - SCOPUS:0027289655

SN - 0014-5793

VL - 324

SP - 296

EP - 300

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

The catalytic domain of human hepatitis delta virus RNA. A proton nuclear magnetic resonance study

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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