The catalytic domain of human hepatitis delta virus RNA. A proton nuclear magnetic resonance study

Bao Shiang Lee, Huey Nan Wu, Tai Huang Huang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have obtained and analyzed the 600 MHz proton NMR spectra of a 74-mer RNA derived from the catalytic domain of hepatitis delta virus genomic RNA (HDV RNA) to determine its secondary structure. Deconvolution of the NMR spectrum obtained at 32°C indicates that part of the 74-mer RNA molecule may exist in multiple conformations in equilibrium. The major conformer contains two A-U base pairs and 14 ± 2 G-C base pairs. It appears to contain no standard G-U base pairs. Our NMR melting study suggests that this conformer has at least two stem-loop regions. One of the regions has been identified to be a tetra-loop. We have assigned five imino proton resonances of the tetraloop stem. Our data is consistent with the pseudoknot model of Perrotta and Been.

Original languageEnglish
Pages (from-to)296-300
Number of pages5
JournalFEBS Letters
Volume324
Issue number3
DOIs
Publication statusPublished - 1993 Jun 21

Keywords

  • Hepatitis delta virus
  • NMR
  • RNA: Structure
  • Ribozyme

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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