Ten novel tetranucleotide microsatellite DNA markers from Asiatic black bear, Ursus thibetanus

Chih Chin Shih; Chuan Chin Huang; Shou Hsien Li; Mei Hsiu Hwang; Ling Ling Lee

doi:10.1007/s10592-009-9830-3

Ten novel tetranucleotide microsatellite DNA markers from Asiatic black bear, Ursus thibetanus

Chih Chin Shih, Chuan Chin Huang, Shou Hsien Li, Mei Hsiu Hwang, Ling Ling Lee^*

^*Corresponding author for this work

Department of Life Science

Research output: Contribution to journal › Comment/debate › peer-review

14 Citations (Scopus)

Abstract

Ten polymorphic microsatellite markers were developed for the endangered Formosan black bear (Ursus thibetanus formosanus) from a partial genomic library enriched for GAAA repeat. Polymorphism of these loci was evaluated in 27 Formosan black bear specimens of unknown relationship. The number of alleles per locus ranged from 5 to 15 and the observed heterozygosity of each locus ranged from 0.556 to 0.889. These loci should provide useful molecular tools to study conservation genetics of the Formosan black bear and other Asiatic black bears.

Original language	English
Pages (from-to)	1845-1847
Number of pages	3
Journal	Conservation Genetics
Volume	10
Issue number	6
DOIs	https://doi.org/10.1007/s10592-009-9830-3
Publication status	Published - 2009 Nov

Keywords

Formosan black bear
Tailed primers
Tetranucleotide microsatellite
Ursus thibetanus

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s10592-009-9830-3

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title = "Ten novel tetranucleotide microsatellite DNA markers from Asiatic black bear, Ursus thibetanus",

abstract = "Ten polymorphic microsatellite markers were developed for the endangered Formosan black bear (Ursus thibetanus formosanus) from a partial genomic library enriched for GAAA repeat. Polymorphism of these loci was evaluated in 27 Formosan black bear specimens of unknown relationship. The number of alleles per locus ranged from 5 to 15 and the observed heterozygosity of each locus ranged from 0.556 to 0.889. These loci should provide useful molecular tools to study conservation genetics of the Formosan black bear and other Asiatic black bears.",

keywords = "Formosan black bear, Tailed primers, Tetranucleotide microsatellite, Ursus thibetanus",

author = "Shih, {Chih Chin} and Huang, {Chuan Chin} and Li, {Shou Hsien} and Hwang, {Mei Hsiu} and Lee, {Ling Ling}",

note = "Funding Information: Acknowledgments We acknowledge Mr. Chieh-Chung Yang in Taiwan Endemic Species Research Institute for providing bear samples. We also thank Ms. Rong-Chien Lin in the Genetic Diversity Laboratory of National Taiwan Normal University for her help with the lab work. This study was funded by the Taiwan Forest Bureau, Council of Agriculture, ROC (95AS-11.1.3-FB-e1) to L-L Lee and supported by Taipei Zoo to C–C Shih.",

year = "2009",

month = nov,

doi = "10.1007/s10592-009-9830-3",

language = "English",

volume = "10",

pages = "1845--1847",

journal = "Conservation Genetics",

issn = "1566-0621",

publisher = "Springer Netherlands",

number = "6",

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TY - JOUR

T1 - Ten novel tetranucleotide microsatellite DNA markers from Asiatic black bear, Ursus thibetanus

AU - Shih, Chih Chin

AU - Huang, Chuan Chin

AU - Li, Shou Hsien

AU - Hwang, Mei Hsiu

AU - Lee, Ling Ling

N1 - Funding Information: Acknowledgments We acknowledge Mr. Chieh-Chung Yang in Taiwan Endemic Species Research Institute for providing bear samples. We also thank Ms. Rong-Chien Lin in the Genetic Diversity Laboratory of National Taiwan Normal University for her help with the lab work. This study was funded by the Taiwan Forest Bureau, Council of Agriculture, ROC (95AS-11.1.3-FB-e1) to L-L Lee and supported by Taipei Zoo to C–C Shih.

PY - 2009/11

Y1 - 2009/11

N2 - Ten polymorphic microsatellite markers were developed for the endangered Formosan black bear (Ursus thibetanus formosanus) from a partial genomic library enriched for GAAA repeat. Polymorphism of these loci was evaluated in 27 Formosan black bear specimens of unknown relationship. The number of alleles per locus ranged from 5 to 15 and the observed heterozygosity of each locus ranged from 0.556 to 0.889. These loci should provide useful molecular tools to study conservation genetics of the Formosan black bear and other Asiatic black bears.

AB - Ten polymorphic microsatellite markers were developed for the endangered Formosan black bear (Ursus thibetanus formosanus) from a partial genomic library enriched for GAAA repeat. Polymorphism of these loci was evaluated in 27 Formosan black bear specimens of unknown relationship. The number of alleles per locus ranged from 5 to 15 and the observed heterozygosity of each locus ranged from 0.556 to 0.889. These loci should provide useful molecular tools to study conservation genetics of the Formosan black bear and other Asiatic black bears.

KW - Formosan black bear

KW - Tailed primers

KW - Tetranucleotide microsatellite

KW - Ursus thibetanus

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U2 - 10.1007/s10592-009-9830-3

DO - 10.1007/s10592-009-9830-3

M3 - Comment/debate

AN - SCOPUS:71349083553

SN - 1566-0621

VL - 10

SP - 1845

EP - 1847

JO - Conservation Genetics

JF - Conservation Genetics

IS - 6

ER -

Ten novel tetranucleotide microsatellite DNA markers from Asiatic black bear, Ursus thibetanus

Abstract

Keywords

ASJC Scopus subject areas

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