Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua

Marian Y. Hu, Katharina Michael, Cornelia M. Kreiss, Meike Stumpp, Sam Dupont, Yung Che Tseng, Magnus Lucassen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

CO2-driven seawater acidification has been demonstrated to enhance intestinal bicarbonate secretion rates in teleosts, leading to an increased release of CaCO3 under simulated ocean acidification scenarios. In this study, we investigated if increasing CO2 levels stimulate the intestinal acid-base regulatory machinery of Atlantic cod (Gadus morhua) and whether temperatures at the upper limit of thermal tolerance stimulate or counteract ion regulatory capacities. Juvenile G. morhua were acclimated for 4 weeks to three CO2 levels (550, 1200, and 2200 μatm) covering present and near-future natural variability, at optimum (10°C) and summer maximum temperature (18°C), respectively. Immunohistochemical analyses revealed the subcellular localization of ion transporters, including Na+/K+-ATPase (NKA), Na+/H+-exchanger 3 (NHE3), Na+/HCO3-cotransporter (NBC1), pendrin-like Cl-/HCO3-exchanger (SLC26a6), V-type H+-ATPase subunit a (VHA), and Cl- channel 3 (CLC3) in epithelial cells of the anterior intestine. At 10°C, proteins and mRNA were generally up-regulated for most transporters in the intestinal epithelium after acclimation to higher CO2 levels. This supports recent findings demonstrating increased intestinal HCO3-secretion rates in response to CO2 induced seawater acidification. At 18°C, mRNA expression and protein concentrations of most ion transporters remained unchanged or were even decreased, suggesting thermal compensation. This response may be energetically favorable to retain blood HCO3-levels to stabilize pHe, but may negatively affect intestinal salt and water resorption of marine teleosts in future oceans.

Original languageEnglish
Article number198
JournalFrontiers in Physiology
Volume7
Issue numberJUN
DOIs
Publication statusPublished - 2016 Jun 2

Fingerprint

Gadus morhua
Ion Transport
Oceans and Seas
Intestinal Secretions
Seawater
Ions
Temperature
Chloride-Bicarbonate Antiporters
Vacuolar Proton-Translocating ATPases
Messenger RNA
Sodium-Hydrogen Antiporter
Proton-Translocating ATPases
Acclimatization
Intestinal Mucosa
Bicarbonates
Intestines
Proteins
Salts
Hot Temperature
Epithelial Cells

Keywords

  • Bicarbonate level
  • Hypercapnia
  • PH regulation
  • Teleost
  • Thermal compensation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua. / Hu, Marian Y.; Michael, Katharina; Kreiss, Cornelia M.; Stumpp, Meike; Dupont, Sam; Tseng, Yung Che; Lucassen, Magnus.

In: Frontiers in Physiology, Vol. 7, No. JUN, 198, 02.06.2016.

Research output: Contribution to journalArticle

Hu, Marian Y. ; Michael, Katharina ; Kreiss, Cornelia M. ; Stumpp, Meike ; Dupont, Sam ; Tseng, Yung Che ; Lucassen, Magnus. / Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua. In: Frontiers in Physiology. 2016 ; Vol. 7, No. JUN.
@article{faf3fde0e7ad434da239fab4a2b1a939,
title = "Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua",
abstract = "CO2-driven seawater acidification has been demonstrated to enhance intestinal bicarbonate secretion rates in teleosts, leading to an increased release of CaCO3 under simulated ocean acidification scenarios. In this study, we investigated if increasing CO2 levels stimulate the intestinal acid-base regulatory machinery of Atlantic cod (Gadus morhua) and whether temperatures at the upper limit of thermal tolerance stimulate or counteract ion regulatory capacities. Juvenile G. morhua were acclimated for 4 weeks to three CO2 levels (550, 1200, and 2200 μatm) covering present and near-future natural variability, at optimum (10°C) and summer maximum temperature (18°C), respectively. Immunohistochemical analyses revealed the subcellular localization of ion transporters, including Na+/K+-ATPase (NKA), Na+/H+-exchanger 3 (NHE3), Na+/HCO3-cotransporter (NBC1), pendrin-like Cl-/HCO3-exchanger (SLC26a6), V-type H+-ATPase subunit a (VHA), and Cl- channel 3 (CLC3) in epithelial cells of the anterior intestine. At 10°C, proteins and mRNA were generally up-regulated for most transporters in the intestinal epithelium after acclimation to higher CO2 levels. This supports recent findings demonstrating increased intestinal HCO3-secretion rates in response to CO2 induced seawater acidification. At 18°C, mRNA expression and protein concentrations of most ion transporters remained unchanged or were even decreased, suggesting thermal compensation. This response may be energetically favorable to retain blood HCO3-levels to stabilize pHe, but may negatively affect intestinal salt and water resorption of marine teleosts in future oceans.",
keywords = "Bicarbonate level, Hypercapnia, PH regulation, Teleost, Thermal compensation",
author = "Hu, {Marian Y.} and Katharina Michael and Kreiss, {Cornelia M.} and Meike Stumpp and Sam Dupont and Tseng, {Yung Che} and Magnus Lucassen",
year = "2016",
month = "6",
day = "2",
doi = "10.3389/fphys.2016.00198",
language = "English",
volume = "7",
journal = "Frontiers in Physiology",
issn = "1664-042X",
publisher = "Frontiers Research Foundation",
number = "JUN",

}

TY - JOUR

T1 - Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua

AU - Hu, Marian Y.

AU - Michael, Katharina

AU - Kreiss, Cornelia M.

AU - Stumpp, Meike

AU - Dupont, Sam

AU - Tseng, Yung Che

AU - Lucassen, Magnus

PY - 2016/6/2

Y1 - 2016/6/2

N2 - CO2-driven seawater acidification has been demonstrated to enhance intestinal bicarbonate secretion rates in teleosts, leading to an increased release of CaCO3 under simulated ocean acidification scenarios. In this study, we investigated if increasing CO2 levels stimulate the intestinal acid-base regulatory machinery of Atlantic cod (Gadus morhua) and whether temperatures at the upper limit of thermal tolerance stimulate or counteract ion regulatory capacities. Juvenile G. morhua were acclimated for 4 weeks to three CO2 levels (550, 1200, and 2200 μatm) covering present and near-future natural variability, at optimum (10°C) and summer maximum temperature (18°C), respectively. Immunohistochemical analyses revealed the subcellular localization of ion transporters, including Na+/K+-ATPase (NKA), Na+/H+-exchanger 3 (NHE3), Na+/HCO3-cotransporter (NBC1), pendrin-like Cl-/HCO3-exchanger (SLC26a6), V-type H+-ATPase subunit a (VHA), and Cl- channel 3 (CLC3) in epithelial cells of the anterior intestine. At 10°C, proteins and mRNA were generally up-regulated for most transporters in the intestinal epithelium after acclimation to higher CO2 levels. This supports recent findings demonstrating increased intestinal HCO3-secretion rates in response to CO2 induced seawater acidification. At 18°C, mRNA expression and protein concentrations of most ion transporters remained unchanged or were even decreased, suggesting thermal compensation. This response may be energetically favorable to retain blood HCO3-levels to stabilize pHe, but may negatively affect intestinal salt and water resorption of marine teleosts in future oceans.

AB - CO2-driven seawater acidification has been demonstrated to enhance intestinal bicarbonate secretion rates in teleosts, leading to an increased release of CaCO3 under simulated ocean acidification scenarios. In this study, we investigated if increasing CO2 levels stimulate the intestinal acid-base regulatory machinery of Atlantic cod (Gadus morhua) and whether temperatures at the upper limit of thermal tolerance stimulate or counteract ion regulatory capacities. Juvenile G. morhua were acclimated for 4 weeks to three CO2 levels (550, 1200, and 2200 μatm) covering present and near-future natural variability, at optimum (10°C) and summer maximum temperature (18°C), respectively. Immunohistochemical analyses revealed the subcellular localization of ion transporters, including Na+/K+-ATPase (NKA), Na+/H+-exchanger 3 (NHE3), Na+/HCO3-cotransporter (NBC1), pendrin-like Cl-/HCO3-exchanger (SLC26a6), V-type H+-ATPase subunit a (VHA), and Cl- channel 3 (CLC3) in epithelial cells of the anterior intestine. At 10°C, proteins and mRNA were generally up-regulated for most transporters in the intestinal epithelium after acclimation to higher CO2 levels. This supports recent findings demonstrating increased intestinal HCO3-secretion rates in response to CO2 induced seawater acidification. At 18°C, mRNA expression and protein concentrations of most ion transporters remained unchanged or were even decreased, suggesting thermal compensation. This response may be energetically favorable to retain blood HCO3-levels to stabilize pHe, but may negatively affect intestinal salt and water resorption of marine teleosts in future oceans.

KW - Bicarbonate level

KW - Hypercapnia

KW - PH regulation

KW - Teleost

KW - Thermal compensation

UR - http://www.scopus.com/inward/record.url?scp=84977664935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84977664935&partnerID=8YFLogxK

U2 - 10.3389/fphys.2016.00198

DO - 10.3389/fphys.2016.00198

M3 - Article

AN - SCOPUS:84977664935

VL - 7

JO - Frontiers in Physiology

JF - Frontiers in Physiology

SN - 1664-042X

IS - JUN

M1 - 198

ER -