Targeted protein quantitation in human body fluids by mass spectrometry

Yi Ting Chen, Wan Rou Liao, Hsueh Ting Wang, Hsiao Wei Chen, Sung Fang Chen*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

5 Citations (Scopus)


Human body fluids (biofluids) contain various proteins, some of which reflect individuals' physiological conditions or predict diseases. Therefore, the analysis of biofluids can provide substantial information on novel biomarkers for clinical diagnosis and prognosis. In the past decades, mass spectrometry (MS)-based technologies have been developed as proteomic strategies not only for the identification of protein biomarkers but also for biomarker verification/validation in body fluids for clinical applications. The main advantage of targeted MS-based methodologies is the accurate and specific simultaneous quantitation of multiple biomarkers with high sensitivity. Here, we review MS-based methodologies that are currently used for the targeted quantitation of protein components in human body fluids, especially in plasma, urine, cerebrospinal fluid, and saliva. In addition, the currently used MS-based methodologies are summarized with a specific focus on applicable clinical sample types, MS configurations, and acquisition modes.

Original languageEnglish
Pages (from-to)2379-2403
Number of pages25
JournalMass Spectrometry Reviews
Issue number6
Publication statusPublished - 2023 Nov 1

ASJC Scopus subject areas

  • Analytical Chemistry
  • Condensed Matter Physics
  • General Biochemistry,Genetics and Molecular Biology
  • Spectroscopy


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