Synthesis of Fe3O4-Chlorophyllin Nanoparticles for Preventing Amyloid Formation by Human Calcitonin

Yu Hsuan Wu, Yu Pei Chang, Chia Chien Hsu, Yi Lun Chen, Pin Han Lin, Ting Wei Hsu, Wei Min Liu, Yun Ju Lai, Mei Yi Liao*, Ling Hsien Tu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The accumulation of amyloid fibrils is crucial in the development of amyloidosis. In addition, some hormone peptides, such as human calcitonin (hCT), have limited therapeutic potential owing to a high tendency of amyloid formation. Nowadays, nanotechnology offers many advantages in various scientific fields and can overcome some of the limitations in the development of traditional therapeutics. In the present study, we applied iron and copper chlorophyllin in the synthesis of iron-oxide nanoparticles to obtain porphyrin derivative-coated nanoparticles, namely, Fe3O4-Chl/Fe and Fe3O4-Chl/Cu, respectively. We observed that the two nanoparticles, especially Fe3O4-Chl/Fe, stabilized hCT and influenced hCT aggregation. To further confirm the applicability of Fe3O4-Chl/Fe, we utilized another benzothiazole-based fluorescent probe to monitor hCT amyloid formation. Several biophysical techniques, including transmission electron microscopy, dynamic light scattering, and isothermal titration calorimetry, were also implemented in our examinations. The fibrillar content of hCT after co-incubation was further determined using Bis-ANS and Nile red assays. The remaining soluble monomeric or oligomeric content of hCT was assessed by gel electrophoresis and biological assays. We believed that our findings would assist in the development of hCT drug formulation. Application of Fe3O4-Chl/Fe can increase the stability and bioactivity of hCT.

Original languageEnglish
Pages (from-to)12598-12608
Number of pages11
JournalACS Applied Nano Materials
Issue number13
Publication statusPublished - 2023 Jul 14


  • amyloid formation
  • drug formulation
  • human calcitonin
  • iron-oxide nanoparticles
  • osteoporosis
  • porphyrin derivative-coated nanoparticles

ASJC Scopus subject areas

  • General Materials Science


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