Survival Motor Neuron Protein Participates in Mouse Germ Cell Development and Spermatogonium Maintenance

Wei Fang Chang, Jie Xu, Tzu Ying Lin, Jing Hsu, Hsiu Mei Hsieh-Li, Yuh Ming Hwu, Ji Long Liu, Chung Hao Lu, Li Ying Sung

Research output: Contribution to journalArticle

Abstract

The defective human survival motor neuron 1 (SMN1) gene leads to spinal muscular atrophy (SMA), the most common genetic cause of infant mortality. We previously reported that loss of SMN results in rapid differentiation of Drosophila germline stem cells and mouse embryonic stem cells (ESCs), indicating that SMN also plays important roles in germ cell development and stem cell biology. Here, we show that in healthy mice, SMN is highly expressed in the gonadal tissues, prepubertal spermatogonia, and adult spermatocytes, whereas low SMN expression is found in differentiated spermatid and sperm. In SMA-like mice, the growth of testis tissues is retarded, accompanied with gamete development abnormalities and loss of the spermatogonia-specific marker. Consistently, knockdown of Smn1 in spermatogonial stem cells (SSCs) leads to a compromised regeneration capacity in vitro and in vivo in transplantation experiments. In SMA-like mice, apoptosis and accumulation of the R-loop structure were significantly elevated, indicating that SMN plays a critical role in the survival of male germ cells. The present work demonstrates that SMN, in addition to its critical roles in neuronal development, participates in mouse germ cell and spermatogonium maintenance.

Original languageEnglish
JournalInternational journal of molecular sciences
Volume21
Issue number3
DOIs
Publication statusPublished - 2020 Jan 25

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Keywords

  • gametogenesis
  • SMN
  • spermatogonium

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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