TY - JOUR
T1 - 1H, 13C and 15N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae
AU - Chen, Sheng Chia
AU - Chang, Chi Fon
AU - Fan, Pei Ju
AU - Cheng, Ya Hsin
AU - Yu, Tsunai
AU - Huang, Tai Huang
N1 - Funding Information:
Acknowledgments The NMR spectra were obtained at High-Field Nuclear Magnetic Resonance Center (HF-NMRC) supported by the National Research Program for Genomic Medicine. This work was supported by a grant from the National Science Council of the Republic of China (NSC 100-2311-B-001-023).
PY - 2013/4
Y1 - 2013/4
N2 - Bacterial cells often use two-component signal transduction systems to regulate genes in response to environmental stimuli. The RstA/RstB system is a two-component regulatory system consisting of the membrane sensor, RstB, and its cognate response regulator RstA. The RstA of Klebsiella pneumoniae consists of a N-terminal receiver domain (NRD, residues 1-119) and a C-terminal DNA-binding domain (DBD, residues 130-236). Phosphorylation of the response regulator induces a conformational change in the regulatory domain of RstA, which results in activation of the effector domain to regulate the downstream genes, including the ferrous iron transport system (Feo), at low-pH condition. Here we report the 1H, 13C and 15N resonance assignments and secondary structure identification of the DBD of RstA from K. pneumoniae as a first step for unraveling the structural and functional relationship of the RstA/RstB two component system.
AB - Bacterial cells often use two-component signal transduction systems to regulate genes in response to environmental stimuli. The RstA/RstB system is a two-component regulatory system consisting of the membrane sensor, RstB, and its cognate response regulator RstA. The RstA of Klebsiella pneumoniae consists of a N-terminal receiver domain (NRD, residues 1-119) and a C-terminal DNA-binding domain (DBD, residues 130-236). Phosphorylation of the response regulator induces a conformational change in the regulatory domain of RstA, which results in activation of the effector domain to regulate the downstream genes, including the ferrous iron transport system (Feo), at low-pH condition. Here we report the 1H, 13C and 15N resonance assignments and secondary structure identification of the DBD of RstA from K. pneumoniae as a first step for unraveling the structural and functional relationship of the RstA/RstB two component system.
KW - DNA-binding
KW - Feo
KW - Ferrous iron transport
KW - Klebsiella pneumoniae
KW - Two component systems
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U2 - 10.1007/s12104-012-9383-0
DO - 10.1007/s12104-012-9383-0
M3 - Article
C2 - 22481468
AN - SCOPUS:84874993963
SN - 1874-2718
VL - 7
SP - 85
EP - 88
JO - Biomolecular NMR Assignments
JF - Biomolecular NMR Assignments
IS - 1
ER -