1H, 13C and 15N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae

Sheng Chia Chen, Chi Fon Chang, Pei Ju Fan, Ya Hsin Cheng, Tsunai Yu, Tai-huang Huang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Bacterial cells often use two-component signal transduction systems to regulate genes in response to environmental stimuli. The RstA/RstB system is a two-component regulatory system consisting of the membrane sensor, RstB, and its cognate response regulator RstA. The RstA of Klebsiella pneumoniae consists of a N-terminal receiver domain (NRD, residues 1-119) and a C-terminal DNA-binding domain (DBD, residues 130-236). Phosphorylation of the response regulator induces a conformational change in the regulatory domain of RstA, which results in activation of the effector domain to regulate the downstream genes, including the ferrous iron transport system (Feo), at low-pH condition. Here we report the 1H, 13C and 15N resonance assignments and secondary structure identification of the DBD of RstA from K. pneumoniae as a first step for unraveling the structural and functional relationship of the RstA/RstB two component system.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalBiomolecular NMR Assignments
Volume7
Issue number1
DOIs
Publication statusPublished - 2013 Apr 1

Fingerprint

Klebsiella pneumoniae
Genes
Signal transduction
Phosphorylation
DNA
Signal Transduction
Proteins
Iron
Chemical activation
Membranes
Sensors
Protein Domains

Keywords

  • DNA-binding
  • Feo
  • Ferrous iron transport
  • Klebsiella pneumoniae
  • Two component systems

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

Cite this

1H, 13C and 15N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae. / Chen, Sheng Chia; Chang, Chi Fon; Fan, Pei Ju; Cheng, Ya Hsin; Yu, Tsunai; Huang, Tai-huang.

In: Biomolecular NMR Assignments, Vol. 7, No. 1, 01.04.2013, p. 85-88.

Research output: Contribution to journalArticle

Chen, Sheng Chia ; Chang, Chi Fon ; Fan, Pei Ju ; Cheng, Ya Hsin ; Yu, Tsunai ; Huang, Tai-huang. / 1H, 13C and 15N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae. In: Biomolecular NMR Assignments. 2013 ; Vol. 7, No. 1. pp. 85-88.
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