1H, 13C and 15N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae

Sheng Chia Chen, Chi Fon Chang, Pei Ju Fan, Ya Hsin Cheng, Tsunai Yu, Tai Huang Huang

Research output: Contribution to journalArticle

1 Citation (Scopus)


Bacterial cells often use two-component signal transduction systems to regulate genes in response to environmental stimuli. The RstA/RstB system is a two-component regulatory system consisting of the membrane sensor, RstB, and its cognate response regulator RstA. The RstA of Klebsiella pneumoniae consists of a N-terminal receiver domain (NRD, residues 1-119) and a C-terminal DNA-binding domain (DBD, residues 130-236). Phosphorylation of the response regulator induces a conformational change in the regulatory domain of RstA, which results in activation of the effector domain to regulate the downstream genes, including the ferrous iron transport system (Feo), at low-pH condition. Here we report the 1H, 13C and 15N resonance assignments and secondary structure identification of the DBD of RstA from K. pneumoniae as a first step for unraveling the structural and functional relationship of the RstA/RstB two component system.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalBiomolecular NMR Assignments
Issue number1
Publication statusPublished - 2013 Apr 1



  • DNA-binding
  • Feo
  • Ferrous iron transport
  • Klebsiella pneumoniae
  • Two component systems

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

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