Subcellular and functional proteomic analysis of the cellular responses induced by Helicobacter pylori

Chia Hsin Chan, Chia Cheng Ko, Jan Gowth Chang, Sung Fang Chen, Ming Shiang Wu, Jaw Town Lin, Lu Ping Chow

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Helicobacter pylori infection is a crucial factor in the pathogenesis of several digestive disorders, including peptic ulcers, chronic gastritis, and gastric cancer. Moreover H. pylori induces disease-specific protein expression in gastric epithelial cells. The aim of the present study was to characterize proteins differentially expressed in H. pylori-infected gastric epithelial AGS cells. An in vitro model was established using a multiplicity of infection of 100 and evaluating the effectiveness of H. pylori infection by functional analyses. Changes in protein patterns were identified using a proteomic approach consisting of two-dimensional fluorescence difference gel electrophoresis and mass spectrometry. The expression of many proteins was found to be altered, and 28 of these were identified and classified as protein synthesis- and folding-related proteins, cytoskeleton proteins, metabolic enzymes, transcription- and translation-related proteins, angiogenesis/metastasis-related proteins, cell communication/signal transduction-related proteins, or others (oxygen-regulated protein and oncoprotein). The expression profiles of eight of these proteins, laminin γ-1 chain precursor, valosin-containing protein, heat shock 70-kDa protein, mitochondrial matrix protein P1, FK506-binding protein 4, T-complex protein 1, enolase α, and 14-3-3 β were further examined in cancerous and paired surrounding normal tissues by immunoblot assay and immunohistochemical staining to identify molecular targets that may be involved in the pathogenesis of H. pylori-induced gastric diseases. On the basis of our results, valosin-containing protein, mitochondrial matrix protein P1, T-complex protein 1, enolase α, and 14-3-3 β may play a crucial role in H. pylori-induced gastric carcinogenesis by mediating antiapoptotic and proliferative responses.

Original languageEnglish
Pages (from-to)702-713
Number of pages12
JournalMolecular and Cellular Proteomics
Volume5
Issue number4
DOIs
Publication statusPublished - 2006 Apr 1

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Helicobacter pylori
Proteomics
Proteins
Stomach
Phosphopyruvate Hydratase
Mitochondrial Proteins
Helicobacter Infections
Epithelial Cells
Two-Dimensional Difference Gel Electrophoresis
Stomach Diseases
HSP70 Heat-Shock Proteins
Oncogene Proteins
Protein Folding
Protein Biosynthesis
Gastritis
Cytoskeleton
Peptic Ulcer
Cell Communication
Signal transduction
Stomach Neoplasms

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Cite this

Subcellular and functional proteomic analysis of the cellular responses induced by Helicobacter pylori. / Chan, Chia Hsin; Ko, Chia Cheng; Chang, Jan Gowth; Chen, Sung Fang; Wu, Ming Shiang; Lin, Jaw Town; Chow, Lu Ping.

In: Molecular and Cellular Proteomics, Vol. 5, No. 4, 01.04.2006, p. 702-713.

Research output: Contribution to journalArticle

Chan, Chia Hsin ; Ko, Chia Cheng ; Chang, Jan Gowth ; Chen, Sung Fang ; Wu, Ming Shiang ; Lin, Jaw Town ; Chow, Lu Ping. / Subcellular and functional proteomic analysis of the cellular responses induced by Helicobacter pylori. In: Molecular and Cellular Proteomics. 2006 ; Vol. 5, No. 4. pp. 702-713.
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