Structure of the SARS Coronavirus Nucleocapsid Protein RNA-binding Dimerization Domain Suggests a Mechanism for Helical Packaging of Viral RNA

Chun Yuan Chen, Chung ke Chang, Yi Wei Chang, Shih Che Sue, Hsin I. Bai, Lilianty Riang, Chwan Deng Hsiao, Tai-huang Huang

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Coronavirus nucleocapsid proteins are basic proteins that encapsulate viral genomic RNA to form part of the virus structure. The nucleocapsid protein of SARS-CoV is highly antigenic and associated with several host-cell interactions. Our previous studies using nuclear magnetic resonance revealed the domain organization of the SARS-CoV nucleocapsid protein. RNA has been shown to bind to the N-terminal domain (NTD), although recently the C-terminal half of the protein has also been implicated in RNA binding. Here, we report that the C-terminal domain (CTD), spanning residues 248-365 (NP248-365), had stronger nucleic acid-binding activity than the NTD. To determine the molecular basis of this activity, we have also solved the crystal structure of the NP248-365 region. Residues 248-280 form a positively charged groove similar to that found in the infectious bronchitis virus (IBV) nucleocapsid protein. Furthermore, the positively charged surface area is larger in the SARS-CoV construct than in the IBV. Interactions between residues 248-280 and the rest of the molecule also stabilize the formation of an octamer in the asymmetric unit. Packing of the octamers in the crystal forms two parallel, basic helical grooves, which may be oligonucleotide attachment sites, and suggests a mechanism for helical RNA packaging in the virus.

Original languageEnglish
Pages (from-to)1075-1086
Number of pages12
JournalJournal of Molecular Biology
Volume368
Issue number4
DOIs
Publication statusPublished - 2007 May 11

Fingerprint

SARS Virus
Nucleocapsid Proteins
Viral RNA
Dimerization
Product Packaging
Protein Binding
Infectious bronchitis virus
RNA
Virus Assembly
Viral Structures
Oligonucleotides
Cell Communication
Nucleic Acids
Proteins
Magnetic Resonance Spectroscopy
Coronavirus nucleocapsid protein
RNA-Binding Motifs

Keywords

  • RNA-binding domain
  • nucleocapsid
  • oligomerization
  • severe acute respiratory syndrome coronavirus
  • viral packaging

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Structure of the SARS Coronavirus Nucleocapsid Protein RNA-binding Dimerization Domain Suggests a Mechanism for Helical Packaging of Viral RNA. / Chen, Chun Yuan; Chang, Chung ke; Chang, Yi Wei; Sue, Shih Che; Bai, Hsin I.; Riang, Lilianty; Hsiao, Chwan Deng; Huang, Tai-huang.

In: Journal of Molecular Biology, Vol. 368, No. 4, 11.05.2007, p. 1075-1086.

Research output: Contribution to journalArticle

Chen, Chun Yuan ; Chang, Chung ke ; Chang, Yi Wei ; Sue, Shih Che ; Bai, Hsin I. ; Riang, Lilianty ; Hsiao, Chwan Deng ; Huang, Tai-huang. / Structure of the SARS Coronavirus Nucleocapsid Protein RNA-binding Dimerization Domain Suggests a Mechanism for Helical Packaging of Viral RNA. In: Journal of Molecular Biology. 2007 ; Vol. 368, No. 4. pp. 1075-1086.
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