Structural dynamics of the two-component response regulator RstA in recognition of promoter DNA element

Yi Chuan Li, Chung Ke Chang, Chi Fon Chang, Ya Hsin Cheng, Pei Ju Fang, Tsunai Yu, Sheng Chia Chen, Yi Ching Li, Chwan Deng Hsiao, Tai Huang Huang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The RstA/RstB system is a bacterial two-component regulatory system consisting of the membrane sensor, RstB and its cognate response regulator (RR) RstA. The RstA of Klebsiella pneumoniae (kpRstA) consists of an N-terminal receiver domain (RD, residues 1-119) and a C-terminal DNA-binding domain (DBD, residues 130-236). Phosphorylation of kpRstA induces dimerization, which allows two kpRstA DBDs to bind to a tandem repeat, called the RstA box, and regulate the expression of downstream genes. Here we report the solution and crystal structures of the free kpRstA RD, DBD and DBD/RstA box DNA complex. The structure of the kpRstA DBD/RstA box complex suggests that the two protomers interact with the RstA box in an asymmetric fashion. Equilibrium binding studies further reveal that the two protomers within the kpRstA dimer bind to the RstA box in a sequential manner. Taken together, our results suggest a binding model where dimerization of the kpRstA RDs provides the platform to allow the first kpRstA DBD protomer to anchor protein-DNA interaction, whereas the second protomer plays a key role in ensuring correct recognition of the RstA box.

Original languageEnglish
Pages (from-to)8777-8788
Number of pages12
JournalNucleic Acids Research
Volume42
Issue number13
DOIs
Publication statusPublished - 2014

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Protein Subunits
DNA
Dimerization
Tandem Repeat Sequences
Klebsiella pneumoniae
Phosphorylation
Gene Expression
Membranes
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Structural dynamics of the two-component response regulator RstA in recognition of promoter DNA element. / Li, Yi Chuan; Chang, Chung Ke; Chang, Chi Fon; Cheng, Ya Hsin; Fang, Pei Ju; Yu, Tsunai; Chen, Sheng Chia; Li, Yi Ching; Hsiao, Chwan Deng; Huang, Tai Huang.

In: Nucleic Acids Research, Vol. 42, No. 13, 2014, p. 8777-8788.

Research output: Contribution to journalArticle

Li, YC, Chang, CK, Chang, CF, Cheng, YH, Fang, PJ, Yu, T, Chen, SC, Li, YC, Hsiao, CD & Huang, TH 2014, 'Structural dynamics of the two-component response regulator RstA in recognition of promoter DNA element', Nucleic Acids Research, vol. 42, no. 13, pp. 8777-8788. https://doi.org/10.1093/nar/gku572
Li, Yi Chuan ; Chang, Chung Ke ; Chang, Chi Fon ; Cheng, Ya Hsin ; Fang, Pei Ju ; Yu, Tsunai ; Chen, Sheng Chia ; Li, Yi Ching ; Hsiao, Chwan Deng ; Huang, Tai Huang. / Structural dynamics of the two-component response regulator RstA in recognition of promoter DNA element. In: Nucleic Acids Research. 2014 ; Vol. 42, No. 13. pp. 8777-8788.
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