Structural, biochemical, and physiological characterization of photosynthesis in leaf-derived cup-shaped galls on Litsea acuminata

Meng Yuan Huang, Wen Dar Huang, Hsueh Mei Chou, Chang Chang Chen, Pei Ju Chen, Yung-Ta Chang, Chi Ming Yang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: The source and sink relationships between insect-induced galls and host plant leaves are interesting. In this research, we collected cup-like galls induced by Bruggmanniella sp. (Diptera: Cecidomyiidae) on host leaves of Litsea acuminata and assessed them to investigate source-sink relationships between galls and host leaves. We characterized several of their photosynthetic characteristics including chlorophyll fluorescence (Fv/Fm), stomatal conductance, and photosynthetic capacity, biochemical components such as total soluble sugar, starches, free amino acids, and soluble proteins. The structural analyses were performed under confocal, light, and scanning electron microscopies. Results: Compared with host leaves, galls exhibited slightly lower chlorophyll fluorescence; however, stomatal conductance and photosynthetic capacity were not detected at all. Galls accumulated higher total soluble sugars and free amino acids but less soluble proteins than host leaves. No stomata was observed on exterior or interior gall surfaces under light or scanning electron microscopy, but their inner surfaces were covered with fungal hyphae. Confocal imagery showed a gradient of chloroplasts distribution between gall outer and inner surfaces. Conclusions: Our results strongly suggest that leaf-derived cecidomyiid galls are a type of chlorophyll-deficient non-leaf green tissue and consists on a novel sink in L. acuminate.

Original languageEnglish
Article number61
JournalBMC Plant Biology
Volume15
Issue number1
DOIs
Publication statusPublished - 2015 Dec 12

Fingerprint

Litsea
galls
photosynthesis
leaves
Cecidomyiidae
chlorophyll
free amino acids
stomatal conductance
scanning electron microscopy
fluorescence
leaf galls
sugars
source-sink relationships
stomata
hyphae
host plants
proteins
chloroplasts
starch
insects

Keywords

  • Cecidomyiidae
  • Chlorophyll fluorescence
  • Gall
  • Litsea acuminate
  • Photosynthesis
  • Sink

ASJC Scopus subject areas

  • Plant Science

Cite this

Structural, biochemical, and physiological characterization of photosynthesis in leaf-derived cup-shaped galls on Litsea acuminata. / Huang, Meng Yuan; Huang, Wen Dar; Chou, Hsueh Mei; Chen, Chang Chang; Chen, Pei Ju; Chang, Yung-Ta; Yang, Chi Ming.

In: BMC Plant Biology, Vol. 15, No. 1, 61, 12.12.2015.

Research output: Contribution to journalArticle

Huang, Meng Yuan ; Huang, Wen Dar ; Chou, Hsueh Mei ; Chen, Chang Chang ; Chen, Pei Ju ; Chang, Yung-Ta ; Yang, Chi Ming. / Structural, biochemical, and physiological characterization of photosynthesis in leaf-derived cup-shaped galls on Litsea acuminata. In: BMC Plant Biology. 2015 ; Vol. 15, No. 1.
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