Stress induced factor 2 regulates arabidopsis stomatal immunity through phosphorylation of the anion channel SLAC1

Ching Chan; Dario Panzeri; Eiji Okuma; Kadri Tõldsepp; Ya Yun Wang; Guan Yu Louh; Tzu Chuan Chin; Yu Hung Yeh; Hung Ling Yeh; Shweta Yekondi; You Huei Huang; Tai Yuan Huang; Tzyy Jen Chiou; Yoshiyuki Murata; Hannes Kollist; Laurent Zimmerli

doi:10.1105/tpc.19.00578

Stress induced factor 2 regulates arabidopsis stomatal immunity through phosphorylation of the anion channel SLAC1

Ching Chan, Dario Panzeri, Eiji Okuma, Kadri Tõldsepp, Ya Yun Wang, Guan Yu Louh, Tzu Chuan Chin, Yu Hung Yeh, Hung Ling Yeh, Shweta Yekondi, You Huei Huang, Tai Yuan Huang, Tzyy Jen Chiou, Yoshiyuki Murata, Hannes Kollist, Laurent Zimmerli^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Upon recognition of microbes, pattern recognition receptors (PRRs) activate pattern-triggered immunity. FLAGELLIN SENSING2 (FLS2) and BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 (BAK1) form a typical PRR complex that senses bacteria. Here, we report that the kinase activity of the malectin-like receptor-like kinase STRESS INDUCED FACTOR 2 (SIF2) is critical for Arabidopsis (Arabidopsis thaliana) resistance to bacteria by regulating stomatal immunity. SIF2 physically associates with the FLS2-BAK1 PRR complex and interacts with and phosphorylates the guard cell SLOW ANION CHANNEL1 (SLAC1), which is necessary for abscisic acid (ABA)-mediated stomatal closure. SIF2 is also required for the activation of ABA-induced S-type anion currents in Arabidopsis protoplasts, and SIF2 is sufficient to activate SLAC1 anion channels in Xenopus oocytes. SIF2-mediated activation of SLAC1 depends on specific phosphorylation of Ser 65. This work reveals that SIF2 functions between the FLS2-BAK1 initial immunity receptor complex and the final actuator SLAC1 in stomatal immunity.

Original language	English
Pages (from-to)	2216-2236
Number of pages	21
Journal	Plant Cell
Volume	32
Issue number	7
DOIs	https://doi.org/10.1105/tpc.19.00578
Publication status	Published - 2020 Jul
Externally published	Yes

ASJC Scopus subject areas

Plant Science

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Chan, C., Panzeri, D., Okuma, E., Tõldsepp, K., Wang, Y. Y., Louh, G. Y., Chin, T. C., Yeh, Y. H., Yeh, H. L., Yekondi, S., Huang, Y. H., Huang, T. Y., Chiou, T. J., Murata, Y., Kollist, H., & Zimmerli, L. (2020). Stress induced factor 2 regulates arabidopsis stomatal immunity through phosphorylation of the anion channel SLAC1. Plant Cell, 32(7), 2216-2236. https://doi.org/10.1105/tpc.19.00578

Chan, C, Panzeri, D, Okuma, E, Tõldsepp, K, Wang, YY, Louh, GY, Chin, TC, Yeh, YH, Yeh, HL, Yekondi, S, Huang, YH, Huang, TY, Chiou, TJ, Murata, Y, Kollist, H & Zimmerli, L 2020, 'Stress induced factor 2 regulates arabidopsis stomatal immunity through phosphorylation of the anion channel SLAC1', Plant Cell, vol. 32, no. 7, pp. 2216-2236. https://doi.org/10.1105/tpc.19.00578

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title = "Stress induced factor 2 regulates arabidopsis stomatal immunity through phosphorylation of the anion channel SLAC1",

abstract = "Upon recognition of microbes, pattern recognition receptors (PRRs) activate pattern-triggered immunity. FLAGELLIN SENSING2 (FLS2) and BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 (BAK1) form a typical PRR complex that senses bacteria. Here, we report that the kinase activity of the malectin-like receptor-like kinase STRESS INDUCED FACTOR 2 (SIF2) is critical for Arabidopsis (Arabidopsis thaliana) resistance to bacteria by regulating stomatal immunity. SIF2 physically associates with the FLS2-BAK1 PRR complex and interacts with and phosphorylates the guard cell SLOW ANION CHANNEL1 (SLAC1), which is necessary for abscisic acid (ABA)-mediated stomatal closure. SIF2 is also required for the activation of ABA-induced S-type anion currents in Arabidopsis protoplasts, and SIF2 is sufficient to activate SLAC1 anion channels in Xenopus oocytes. SIF2-mediated activation of SLAC1 depends on specific phosphorylation of Ser 65. This work reveals that SIF2 functions between the FLS2-BAK1 initial immunity receptor complex and the final actuator SLAC1 in stomatal immunity.",

author = "Ching Chan and Dario Panzeri and Eiji Okuma and Kadri T{\~o}ldsepp and Wang, {Ya Yun} and Louh, {Guan Yu} and Chin, {Tzu Chuan} and Yeh, {Yu Hung} and Yeh, {Hung Ling} and Shweta Yekondi and Huang, {You Huei} and Huang, {Tai Yuan} and Chiou, {Tzyy Jen} and Yoshiyuki Murata and Hannes Kollist and Laurent Zimmerli",

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doi = "10.1105/tpc.19.00578",

language = "English",

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AU - Chan, Ching

AU - Panzeri, Dario

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AU - Tõldsepp, Kadri

AU - Wang, Ya Yun

AU - Louh, Guan Yu

AU - Chin, Tzu Chuan

AU - Yeh, Yu Hung

AU - Yeh, Hung Ling

AU - Yekondi, Shweta

AU - Huang, You Huei

AU - Huang, Tai Yuan

AU - Chiou, Tzyy Jen

AU - Murata, Yoshiyuki

AU - Kollist, Hannes

AU - Zimmerli, Laurent

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AB - Upon recognition of microbes, pattern recognition receptors (PRRs) activate pattern-triggered immunity. FLAGELLIN SENSING2 (FLS2) and BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 (BAK1) form a typical PRR complex that senses bacteria. Here, we report that the kinase activity of the malectin-like receptor-like kinase STRESS INDUCED FACTOR 2 (SIF2) is critical for Arabidopsis (Arabidopsis thaliana) resistance to bacteria by regulating stomatal immunity. SIF2 physically associates with the FLS2-BAK1 PRR complex and interacts with and phosphorylates the guard cell SLOW ANION CHANNEL1 (SLAC1), which is necessary for abscisic acid (ABA)-mediated stomatal closure. SIF2 is also required for the activation of ABA-induced S-type anion currents in Arabidopsis protoplasts, and SIF2 is sufficient to activate SLAC1 anion channels in Xenopus oocytes. SIF2-mediated activation of SLAC1 depends on specific phosphorylation of Ser 65. This work reveals that SIF2 functions between the FLS2-BAK1 initial immunity receptor complex and the final actuator SLAC1 in stomatal immunity.

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Stress induced factor 2 regulates arabidopsis stomatal immunity through phosphorylation of the anion channel SLAC1

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