Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B

Chun Li Su, Chun Chun Cheng, Mao Tsun Lin, Hsiao Chun Yeh, Meng Chou Lee, Jenq Chang Lee, Shen Jeu Won

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The staphylococcal enterotoxins produced by Staphylococcus aureus are associated with pyrogenic response in humans and primates. This study investigates the role of NADPH oxidase and nuclear factor-kappa B (NF-κB) on enterotoxin staphylococcal enterotoxin C1 (SEC1)-induced pyrogenic cytokine production in human peripheral blood mononuclear cells (PBMC). The results indicate that the febrile response to the supernatant fluids of SEC1-stimulated PBMC in rabbits was in parallel with the levels of interleukin-1β and interleukin-6 in the supernatants. The release of interleukin-1β and interleukin-6, nuclear translocation of NF-κB and its DNA binding activity in the SEC1-stimulated PBMC were time-dependent and were completely eliminated by pyrrolidine dithiocarbamate or SN-50 (NF-κB inhibitors). The release of reactive oxygen species in the supernatants and translocation of the NADPH oxidase p47phox subunit to the plasma membrane of SEC1-stimulated PBMC were time-dependent. Administration of apocynin (NADPH oxidase inhibitor) attenuated the febrile response to the supernatants in rabbits and decreased the translocation of NADPH oxidase p47phox subunit and NF-κB activity in the SEC1-stimulated PBMC, and suppressed reactive oxygen species and pyrogenic cytokine production in the supernatants. Taken together, SEC1 may act through an NADPH oxidase mechanism to release reactive oxygen species, which activate NF-κB in PBMC to stimulate the synthesis of pyrogenic cytokines that trigger a fever response in rabbits.

Original languageEnglish
Pages (from-to)3633-3645
Number of pages13
JournalFEBS Journal
Volume274
Issue number14
DOIs
Publication statusPublished - 2007 Jul 1

Fingerprint

NF-kappa B
NADPH Oxidase
Enterotoxins
Blood Cells
Blood
Cytokines
Reactive Oxygen Species
Fever
Rabbits
Interleukin-1
Interleukin-6
Cell membranes
Primates
Staphylococcus aureus
Cell Membrane
Fluids
DNA

Keywords

  • Human peripheral blood mononuclear cells
  • NADPH oxidase
  • NF-κB
  • Pyrogenic cytokine
  • Staphylococcal enterotoxin C1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B. / Su, Chun Li; Cheng, Chun Chun; Lin, Mao Tsun; Yeh, Hsiao Chun; Lee, Meng Chou; Lee, Jenq Chang; Won, Shen Jeu.

In: FEBS Journal, Vol. 274, No. 14, 01.07.2007, p. 3633-3645.

Research output: Contribution to journalArticle

Su, Chun Li ; Cheng, Chun Chun ; Lin, Mao Tsun ; Yeh, Hsiao Chun ; Lee, Meng Chou ; Lee, Jenq Chang ; Won, Shen Jeu. / Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B. In: FEBS Journal. 2007 ; Vol. 274, No. 14. pp. 3633-3645.
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