Stability study for magnetic reagent assaying Hb and HbA1c

Wen Pin Hsieh; J. J. Chieh; C. C. Yang; S. Y. Yang; Po Yu Chen; Yu Hao Huang; Y. W. Hong; H. E. Horng

doi:10.1016/j.jmmm.2012.08.029

Stability study for magnetic reagent assaying Hb and HbA1c

Wen Pin Hsieh, J. J. Chieh, C. C. Yang, S. Y. Yang, Po Yu Chen, Yu Hao Huang, Y. W. Hong, H. E. Horng^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe ₃O ₄ magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 °C. This means that the reagents synthesized in this work are promising for practical applications.

Original language	English
Pages (from-to)	147-150
Number of pages	4
Journal	Journal of Magnetism and Magnetic Materials
Volume	326
DOIs	https://doi.org/10.1016/j.jmmm.2012.08.029
Publication status	Published - 2013 Jan

Keywords

Hb
HbA1c
Immunoassay
Magnetic particle
Stability

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics

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10.1016/j.jmmm.2012.08.029

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@article{da1e47f90bb84ed591b7d0ace8a20b60,

title = "Stability study for magnetic reagent assaying Hb and HbA1c",

abstract = "Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe 3O 4 magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 °C. This means that the reagents synthesized in this work are promising for practical applications.",

keywords = "Hb, HbA1c, Immunoassay, Magnetic particle, Stability",

author = "Hsieh, {Wen Pin} and Chieh, {J. J.} and Yang, {C. C.} and Yang, {S. Y.} and Chen, {Po Yu} and Huang, {Yu Hao} and Hong, {Y. W.} and Horng, {H. E.}",

note = "Funding Information: This work was supported by the National Science Council of Taiwan under Grant nos. NSC100-2112-M003-010 , NSC 100-2120-M-002-015, NSC101-2120-M168-001, and by the Department of Health under Grant nos. DOH100-TD-N-111-008 , DOH101-TD-N-111-004, and by the Ministry of Economic Affairs, Taiwan under Grant nos. 1Z1000408 , 1Z970688, 1Z0990415 (SBIR), and S09800226-203 (JAID).",

year = "2013",

month = jan,

doi = "10.1016/j.jmmm.2012.08.029",

language = "English",

volume = "326",

pages = "147--150",

journal = "Journal of Magnetism and Magnetic Materials",

issn = "0304-8853",

publisher = "Elsevier",

}

TY - JOUR

T1 - Stability study for magnetic reagent assaying Hb and HbA1c

AU - Hsieh, Wen Pin

AU - Chieh, J. J.

AU - Yang, C. C.

AU - Yang, S. Y.

AU - Chen, Po Yu

AU - Huang, Yu Hao

AU - Hong, Y. W.

AU - Horng, H. E.

N1 - Funding Information: This work was supported by the National Science Council of Taiwan under Grant nos. NSC100-2112-M003-010 , NSC 100-2120-M-002-015, NSC101-2120-M168-001, and by the Department of Health under Grant nos. DOH100-TD-N-111-008 , DOH101-TD-N-111-004, and by the Ministry of Economic Affairs, Taiwan under Grant nos. 1Z1000408 , 1Z970688, 1Z0990415 (SBIR), and S09800226-203 (JAID).

PY - 2013/1

Y1 - 2013/1

N2 - Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe 3O 4 magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 °C. This means that the reagents synthesized in this work are promising for practical applications.

AB - Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe 3O 4 magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 °C. This means that the reagents synthesized in this work are promising for practical applications.

KW - Hb

KW - HbA1c

KW - Immunoassay

KW - Magnetic particle

KW - Stability

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U2 - 10.1016/j.jmmm.2012.08.029

DO - 10.1016/j.jmmm.2012.08.029

M3 - Article

AN - SCOPUS:84868676759

SN - 0304-8853

VL - 326

SP - 147

EP - 150

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

ER -

Stability study for magnetic reagent assaying Hb and HbA1c

Abstract

Keywords

ASJC Scopus subject areas

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