Stability study for magnetic reagent assaying Hb and HbA1c

Wen Pin Hsieh, Jen-Je Chieh, C. C. Yang, Shieh-Yueh Yang, Po Yu Chen, Yu Hao Huang, Y. W. Hong, Herng-Er Horng

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe 3O 4 magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 °C. This means that the reagents synthesized in this work are promising for practical applications.

    Original languageEnglish
    Pages (from-to)147-150
    Number of pages4
    JournalJournal of Magnetism and Magnetic Materials
    Volume326
    DOIs
    Publication statusPublished - 2013 Jan 1

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    reagents
    Assays
    Bioactivity
    Antibodies
    Particle size
    immunoassay
    antibodies
    curves

    Keywords

    • Hb
    • HbA1c
    • Immunoassay
    • Magnetic particle
    • Stability

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Condensed Matter Physics

    Cite this

    Stability study for magnetic reagent assaying Hb and HbA1c. / Hsieh, Wen Pin; Chieh, Jen-Je; Yang, C. C.; Yang, Shieh-Yueh; Chen, Po Yu; Huang, Yu Hao; Hong, Y. W.; Horng, Herng-Er.

    In: Journal of Magnetism and Magnetic Materials, Vol. 326, 01.01.2013, p. 147-150.

    Research output: Contribution to journalArticle

    Hsieh, Wen Pin ; Chieh, Jen-Je ; Yang, C. C. ; Yang, Shieh-Yueh ; Chen, Po Yu ; Huang, Yu Hao ; Hong, Y. W. ; Horng, Herng-Er. / Stability study for magnetic reagent assaying Hb and HbA1c. In: Journal of Magnetism and Magnetic Materials. 2013 ; Vol. 326. pp. 147-150.
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    AU - Chieh, Jen-Je

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    AU - Yang, Shieh-Yueh

    AU - Chen, Po Yu

    AU - Huang, Yu Hao

    AU - Hong, Y. W.

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    AB - Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe 3O 4 magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 °C. This means that the reagents synthesized in this work are promising for practical applications.

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