Stability study for magnetic reagent assaying Hb and HbA1c

Wen Pin Hsieh, J. J. Chieh, C. C. Yang, S. Y. Yang, Po Yu Chen, Yu Hao Huang, Y. W. Hong, H. E. Horng

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe 3O 4 magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 °C. This means that the reagents synthesized in this work are promising for practical applications.

Original languageEnglish
Pages (from-to)147-150
Number of pages4
JournalJournal of Magnetism and Magnetic Materials
Volume326
DOIs
Publication statusPublished - 2013 Jan 1

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Keywords

  • Hb
  • HbA1c
  • Immunoassay
  • Magnetic particle
  • Stability

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

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