Spz1, a novel bHLH-Zip protein, is specifically expressed in testis

Shih Hsien Hsu; Huey Wen Shyu; Hsiu Mei Hsieh-Li; Hung Li

doi:10.1016/S0925-4773(00)00513-X

Spz1, a novel bHLH-Zip protein, is specifically expressed in testis

Shih Hsien Hsu, Huey Wen Shyu, Hsiu Mei Hsieh-Li, Hung Li^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

We isolated a novel bHLH-Zip gene designated Spz1 from a mouse testis cDNA library. Spz1 is expressed specifically in the testis and epididymis. Immunofluorescence staining detected Spz1 protein in the nuclei of LFG6 Leydig cells. The ability of Spz1 protein to bind to the bHLH consensus-binding site, the E-box, was confirmed by EMSA, and a 9-bp asymmetric target site was identified by random selection and PCR amplification. Hormonal regulation of Spz1 was investigated and downregulation of Spz1 expression by testosterone and retinoic acid was found. This nuclear transcription factor may play a crucial role in spermatogenesis by regulating cell proliferation or differentiation through binding to specific DNA sequences like other bHLH-Zip molecules.

Original language	English
Pages (from-to)	177-187
Number of pages	11
Journal	Mechanisms of Development
Volume	100
Issue number	2
DOIs	https://doi.org/10.1016/S0925-4773(00)00513-X
Publication status	Published - 2001
Externally published	Yes

Keywords

Basic helix-loop-helix
Leucine zipper
Mouse
Spermatogenesis
Testis

ASJC Scopus subject areas

Embryology
Developmental Biology

Access to Document

10.1016/S0925-4773(00)00513-X

Cite this

@article{15e2fe207b1f4cdaacec26b9b035c764,

title = "Spz1, a novel bHLH-Zip protein, is specifically expressed in testis",

abstract = "We isolated a novel bHLH-Zip gene designated Spz1 from a mouse testis cDNA library. Spz1 is expressed specifically in the testis and epididymis. Immunofluorescence staining detected Spz1 protein in the nuclei of LFG6 Leydig cells. The ability of Spz1 protein to bind to the bHLH consensus-binding site, the E-box, was confirmed by EMSA, and a 9-bp asymmetric target site was identified by random selection and PCR amplification. Hormonal regulation of Spz1 was investigated and downregulation of Spz1 expression by testosterone and retinoic acid was found. This nuclear transcription factor may play a crucial role in spermatogenesis by regulating cell proliferation or differentiation through binding to specific DNA sequences like other bHLH-Zip molecules.",

keywords = "Basic helix-loop-helix, Leucine zipper, Mouse, Spermatogenesis, Testis",

author = "Hsu, {Shih Hsien} and Shyu, {Huey Wen} and Hsieh-Li, {Hsiu Mei} and Hung Li",

note = "Funding Information: We would like to thank Drs W.W. Branford, S.S. Potter and K.B. Choo for their critical reading of the manuscript. This work was supported in part by a research grant NSC-89-2311-B-001-214 from the National Science Council, Taiwan.",

year = "2001",

doi = "10.1016/S0925-4773(00)00513-X",

language = "English",

volume = "100",

pages = "177--187",

journal = "Mechanisms of Development",

issn = "0925-4773",

publisher = "John Wiley & Sons Inc.",

number = "2",

}

TY - JOUR

T1 - Spz1, a novel bHLH-Zip protein, is specifically expressed in testis

AU - Hsu, Shih Hsien

AU - Shyu, Huey Wen

AU - Hsieh-Li, Hsiu Mei

AU - Li, Hung

N1 - Funding Information: We would like to thank Drs W.W. Branford, S.S. Potter and K.B. Choo for their critical reading of the manuscript. This work was supported in part by a research grant NSC-89-2311-B-001-214 from the National Science Council, Taiwan.

PY - 2001

Y1 - 2001

N2 - We isolated a novel bHLH-Zip gene designated Spz1 from a mouse testis cDNA library. Spz1 is expressed specifically in the testis and epididymis. Immunofluorescence staining detected Spz1 protein in the nuclei of LFG6 Leydig cells. The ability of Spz1 protein to bind to the bHLH consensus-binding site, the E-box, was confirmed by EMSA, and a 9-bp asymmetric target site was identified by random selection and PCR amplification. Hormonal regulation of Spz1 was investigated and downregulation of Spz1 expression by testosterone and retinoic acid was found. This nuclear transcription factor may play a crucial role in spermatogenesis by regulating cell proliferation or differentiation through binding to specific DNA sequences like other bHLH-Zip molecules.

AB - We isolated a novel bHLH-Zip gene designated Spz1 from a mouse testis cDNA library. Spz1 is expressed specifically in the testis and epididymis. Immunofluorescence staining detected Spz1 protein in the nuclei of LFG6 Leydig cells. The ability of Spz1 protein to bind to the bHLH consensus-binding site, the E-box, was confirmed by EMSA, and a 9-bp asymmetric target site was identified by random selection and PCR amplification. Hormonal regulation of Spz1 was investigated and downregulation of Spz1 expression by testosterone and retinoic acid was found. This nuclear transcription factor may play a crucial role in spermatogenesis by regulating cell proliferation or differentiation through binding to specific DNA sequences like other bHLH-Zip molecules.

KW - Basic helix-loop-helix

KW - Leucine zipper

KW - Mouse

KW - Spermatogenesis

KW - Testis

UR - http://www.scopus.com/inward/record.url?scp=0035141829&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035141829&partnerID=8YFLogxK

U2 - 10.1016/S0925-4773(00)00513-X

DO - 10.1016/S0925-4773(00)00513-X

M3 - Article

C2 - 11165476

AN - SCOPUS:0035141829

SN - 0925-4773

VL - 100

SP - 177

EP - 187

JO - Mechanisms of Development

JF - Mechanisms of Development

IS - 2

ER -

Spz1, a novel bHLH-Zip protein, is specifically expressed in testis

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this