Spatial expression and functional flexibility of monocarboxylate transporter isoforms in the zebrafish brain

Yung-Che Tseng, Zhi Jie Kao, Sian Tai Liu, Ruo Dong Chen, Pung Pung Hwang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The present study provides in vivo evidence to prove the functional plasticity of monocarboxylate transporters (MCTs) in brains of vertebrates using zebrafish (Danio rerio) as a model. In the mammalian central nervous system (CNS), energy demands are largely met by oxidation of glucose. In recent studies, in addition to glucose, lactate is also considered an energy substrate for the CNS. Astrocytes were demonstrated to play an important role in transporting lactate as metabolic substrate from capillaries to neurons through monocarboxylate transporters (MCTs). The present study was to use zebrafish as an in vivo model to test the hypothesis of whether the various MCT homologs play differential roles in the development and functioning of the CNS. Using RT-PCR and double in situ hybridization coupling with immunocytochemical staining experiments, zebrafish MCTs1-4 were all found to be expressed in brains of embryos, and were further elucidated to be localized in both neurons and astrocytes. Loss-of-functions by morpholino knockdown further provided in vivo evidences to infer that zMCTs1, -2, and -4 may be involved in metabolite transport and functioning in the developing brain. Subsequent rescue experiments with capped mRNAs of specific isoforms further indicated that zMCT2 is an indispensable monocarboxylate-transporting route for CNS development and function in zebrafish. This information is essential for identifying proper candidates of MCT isoforms that are involved in the development and functioning of the CNS.

Original languageEnglish
Pages (from-to)106-118
Number of pages13
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume165
Issue number2
DOIs
Publication statusPublished - 2013 Jun 1

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Neurology
Zebrafish
Brain
Protein Isoforms
Central Nervous System
Astrocytes
Neurons
Lactic Acid
RNA Isoforms
Glucose
Morpholinos
Substrates
Metabolites
In Situ Hybridization
Plasticity
Vertebrates
Embryonic Structures
Experiments
Staining and Labeling
Polymerase Chain Reaction

Keywords

  • CNS
  • Development
  • Monocarboxylate transporter
  • Zebrafish

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Molecular Biology

Cite this

Spatial expression and functional flexibility of monocarboxylate transporter isoforms in the zebrafish brain. / Tseng, Yung-Che; Kao, Zhi Jie; Liu, Sian Tai; Chen, Ruo Dong; Hwang, Pung Pung.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 165, No. 2, 01.06.2013, p. 106-118.

Research output: Contribution to journalArticle

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