Soy-based multiple amino acid oral supplementation increases the anti-sarcoma effect of cyclophosphamide

Chien An Yao, Chin Chu Chen, Nai Phog Wang, Chiang Ting Chien

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The use of a mixture of amino acids caused a selective apoptosis induction against a variety of tumor cell lines, reduced the adverse effects of anti-cancer drugs and increased the sensitivity of tumor cells to chemotherapeutic agents. We evaluated the effects and underlying mechanisms of soy-derived multiple amino acids’ oral supplementation on the therapeutic efficacy of low-dose cyclophosphamide (CTX) and on tumor growth, apoptosis, and autophagy in severe combined immunodeficiency (SCID) mice that were injected with sarcoma-180 (S-180) cells. 3-methyladenine or siRNA knockdown of Atg5 was used to evaluate its effect on sarcoma growth. A comparison of mice with implanted sarcoma cells, CTX, and oral saline and mice with implanted sarcoma cells, CTX, and an oral soy-derived multiple amino acid supplement indicated that the soy-derived multiple amino acid supplement significantly decreased overall sarcoma growth, increased the Bax/Bcl-2 ratio, caspase 3 expression, and apoptosis, and depressed LC3 II-mediated autophagy. Treatment with 3-methyladenine or Atg5 siRNA elicited similar responses as CTX plus soy-derived multiple amino acid in downregulating autophagy and upregulating apoptosis. A low dose of CTX combined with an oral soy-derived multiple amino acid supplement had a potent anti-tumor effect mediated through downregulation of autophagy and upregulation of apoptosis.

Original languageEnglish
Article number192
JournalNutrients
Volume8
Issue number4
DOIs
Publication statusPublished - 2016 Apr 1

Keywords

  • Apoptosis
  • Atg5
  • Autophagy
  • Chemotherapy
  • Mice
  • Soy-based amino acids

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

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