Sox3 expression identifies neural progenitors in persistent neonatal and adult mouse forebrain germinative zones

Tsu-Wei Wang, Gregory P. Stromberg, Justin T. Whitney, Nathan W. Brower, Michael W. Klymkowsky, Jack M. Parent

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Neural precursors persist throughout life in the rodent forebrain subventricular zone (SVZ) and hippocampal dentate gyrus. The regulation of persistent neural stem cells is poorly understood, in part because of the lack of neural progenitor markers. The Sox B1 subfamily of HMG-box transcription factors (Sox1-3) is expressed by precursors in the embryonic nervous system, where these factors maintain neural progenitors in an undifferentiated state while suppressing neuronal differentiation. Sox2 expression persists in germinative zones of the adult rodent brain, but Sox3 expression in the postnatal brain remains largely unexplored. Here we examine Sox3 expression in the neonatal and adult mouse brain to gain insight into its potential involvement in regulating persistent neural stem cells and neurogenesis. We also investigate Sox3 expression during expansion and neural differentiation of postnatal mouse SVZ neural stem cell and human embryonic stem cell (hESC) cultures. We find that Sox3 is expressed transiently by proliferating and differentiating neural progenitors in the SVZ-olfactory bulb pathway and dentate gyrus. Sox3 immunoreactivity also persists in specific postmitotic neuronal populations. In vitro, high Sox3 protein expression levels in undifferentiated, SVZ-derived neurospheres decline markedly with differentiation. Sox3 immunoreactivity in hESCs appears upon differentiation to neural progenitors and then decreases as cells differentiate further into neurons. These findings suggest that Sox3 labels specific stages of hESC-derived and murine neonatal and adult neural progenitors and are consistent with a role for Sox3 in neural stem cell maintenance. Persistent Sox3 expression in some mature neuronal populations suggests additional undefined roles for Sox3 in neuronal function.

Original languageEnglish
Pages (from-to)88-100
Number of pages13
JournalJournal of Comparative Neurology
Volume497
Issue number1
DOIs
Publication statusPublished - 2006 Jul 1

Fingerprint

Neural Stem Cells
Lateral Ventricles
Prosencephalon
Dentate Gyrus
Transcription Factor 7-Like 1 Protein
Rodentia
Brain
Parahippocampal Gyrus
Olfactory Pathways
Olfactory Bulb
Neurogenesis
Nervous System
Population
Cell Culture Techniques
Maintenance
Neurons
Human Embryonic Stem Cells
Proteins

Keywords

  • Dentate gyrus
  • Neural stem cell
  • Neurogenesis
  • Sox3
  • Subventricular zone

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sox3 expression identifies neural progenitors in persistent neonatal and adult mouse forebrain germinative zones. / Wang, Tsu-Wei; Stromberg, Gregory P.; Whitney, Justin T.; Brower, Nathan W.; Klymkowsky, Michael W.; Parent, Jack M.

In: Journal of Comparative Neurology, Vol. 497, No. 1, 01.07.2006, p. 88-100.

Research output: Contribution to journalArticle

Wang, Tsu-Wei ; Stromberg, Gregory P. ; Whitney, Justin T. ; Brower, Nathan W. ; Klymkowsky, Michael W. ; Parent, Jack M. / Sox3 expression identifies neural progenitors in persistent neonatal and adult mouse forebrain germinative zones. In: Journal of Comparative Neurology. 2006 ; Vol. 497, No. 1. pp. 88-100.
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