SMN is required for the maintenance of embryonic stem cells and neuronal differentiation in mice

Wei Fang Chang, Jie Xu, Chia Chun Chang, Shang Hsun Yang, Hsin Yang Li, Hsiu Mei Hsieh-Li, Mong Hsun Tsai, Shinn Chih Wu, Winston T.K. Cheng, Ji Long Liu, Li Ying Sung

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Survival motor neuron (SMN) is the determining factor in spinal muscular atrophy, the most common genetic cause of childhood mortality. We have previously found that SMN regulates stem cell division, proliferation and differentiation in Drosophila. However, it is unknown whether a similar effect exists in vertebrates. Here, we show that SMN is enriched in highly proliferative embryonic stem cells (ESCs) in mice and reduction of SMN impairs the pluripotency of ESCs. Moreover, we find that SMN reduction activates ERK signaling and affects neuronal differentiation in vitro. Teratomas with reduced SMN grow more slowly and show weaker signals of neuronal differentiation than those with a normal level of SMN. Finally, we show that over-expression of SMN is protective for ESCs from retinoic acid-induced differentiation. Taken together, our results suggest that SMN plays a role in the maintenance of pluripotent ESCs and neuronal differentiation in mice.

Original languageEnglish
Pages (from-to)1539-1553
Number of pages15
JournalBrain Structure and Function
Volume220
Issue number3
DOIs
Publication statusPublished - 2015 May 1

Keywords

  • ERK signaling
  • Embryonic stem cell (ESC)
  • Neuronal differentiation
  • Survival motor neuron (SMN)

ASJC Scopus subject areas

  • Anatomy
  • Neuroscience(all)
  • Histology

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