Site-directed mutagenesis improves the thermostability of a recombinant Picrophilus torridus trehalose synthase and efficiency for the production of trehalose from sweet potato starch

Hsin Hung Chou, Shu Wei Chang, Guan-Chiun Lee, Yi Shan Chen, Tzunuan Yeh, Casimir C. Akoh, Jei Fu Shaw

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A new recombinant Picrophilus torridus TSase (PTTS) has the catalytic ability for the conversion of maltose to trehalose by intramolecular transglucosylation. For industrial applications, the high thermostability of the enzyme would be the most important property for reducing the microbial contamination and lower the production cost. Therefore, in this study, we substituted ten selected proline residues of PTTS which differ from two well-known thermostable TSases. Interestingly, we found that the N503 mutant type, namely N503P-PTTS, showed about 39% higher relative activity than that of the wild type at 65 °C for 120 min. The trehalose yield of mutant N503P-PTTS was 1.3-fold higher than that of the wild type with sweet potato starch as substrate at 50 °C for 4 h. This suggests that the proline site substitution technology used in this study is useful for altering enzyme properties and catalytic efficiency for possible industrial applications.

Original languageEnglish
Pages (from-to)1017-1022
Number of pages6
JournalFood Chemistry
Volume119
Issue number3
DOIs
Publication statusPublished - 2010 Apr 1

Fingerprint

Thermoplasmales
Ipomoea batatas
Mutagenesis
Trehalose
site-directed mutagenesis
trehalose
Site-Directed Mutagenesis
thermal stability
Proline
Starch
Industrial applications
Maltose
industrial applications
Enzymes
proline
Contamination
Substitution reactions
mutants
microbial contamination
Substrates

Keywords

  • Amylase
  • Maltose
  • Mutation
  • Proline
  • Sweet potato
  • Thermostability
  • Trehalose
  • Trehalose synthase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Cite this

Site-directed mutagenesis improves the thermostability of a recombinant Picrophilus torridus trehalose synthase and efficiency for the production of trehalose from sweet potato starch. / Chou, Hsin Hung; Chang, Shu Wei; Lee, Guan-Chiun; Chen, Yi Shan; Yeh, Tzunuan; Akoh, Casimir C.; Shaw, Jei Fu.

In: Food Chemistry, Vol. 119, No. 3, 01.04.2010, p. 1017-1022.

Research output: Contribution to journalArticle

Chou, Hsin Hung ; Chang, Shu Wei ; Lee, Guan-Chiun ; Chen, Yi Shan ; Yeh, Tzunuan ; Akoh, Casimir C. ; Shaw, Jei Fu. / Site-directed mutagenesis improves the thermostability of a recombinant Picrophilus torridus trehalose synthase and efficiency for the production of trehalose from sweet potato starch. In: Food Chemistry. 2010 ; Vol. 119, No. 3. pp. 1017-1022.
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