Single-layer graphene based SPR biochips for tuberculosis bacillus detection

Nan Fu Chiu; Teng Yi Huang; Chun Chuan Kuo; Wei Che Lee; Min Hua Hsieh; Hsin Chih Lai

doi:10.1117/12.922698

Single-layer graphene based SPR biochips for tuberculosis bacillus detection

Nan Fu Chiu^*
, Teng Yi Huang
, Chun Chuan Kuo
, Wei Che Lee
, Min Hua Hsieh
, Hsin Chih Lai

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

22 Citations (Scopus)

Abstract

This paper is intended to demonstrate a facile and effective method to construct single layer graphene films onto the self-assembled monolayer (SAM) at Au electrodes based surface plasmon resonance (SPR) biochips integrated loop-mediated isothermal amplification (LAMP) for tuberculosis bacillus (TB) detection. It is a novel Au-SAM-graphene nanocomposites and taking advantages of the striking properties of both graphene and Au film, fundamental understanding in hybrid material manipulation and new electrochemical properties can be obtained. The sensitivity of TB detection in the LAMP-based assay for the amplification of the Insertion Sequence 6110 (IS6110) samples was determined by a single-layer graphene/Au thin film and compared with that of a conventional Au/Cr-based SPR chips. The results show that a graphene/Au SPR offers a potentially powerful assay, with a highly sensitive analysis, that may be applicable as an important tool for bio-marker detection.

Original language	English
Title of host publication	Biophotonics
Subtitle of host publication	Photonic Solutions for Better Health Care III
DOIs	https://doi.org/10.1117/12.922698
Publication status	Published - 2012
Event	Biophotonics: Photonic Solutions for Better Health Care III - Brussels, Belgium Duration: 2012 Apr 16 → 2012 Apr 19

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	8427
ISSN (Print)	1605-7422

Other

Other	Biophotonics: Photonic Solutions for Better Health Care III
Country/Territory	Belgium
City	Brussels
Period	2012/04/16 → 2012/04/19

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biosensor
Graphene
Insertion Sequence 6110 (IS6110)
Loop-mediated isothermal amplification (LAMP)
Self-assembled monolayer (SAM)
Surface plasmon resonance (SPR)
Tuberculosis bacillus (TB)

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.922698

Cite this

Single-layer graphene based SPR biochips for tuberculosis bacillus detection. / Chiu, Nan Fu; Huang, Teng Yi; Kuo, Chun Chuan et al.
Biophotonics: Photonic Solutions for Better Health Care III. 2012. 84273M (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8427).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Chiu, NF, Huang, TY, Kuo, CC, Lee, WC, Hsieh, MH & Lai, HC 2012, Single-layer graphene based SPR biochips for tuberculosis bacillus detection. in Biophotonics: Photonic Solutions for Better Health Care III., 84273M, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8427, Biophotonics: Photonic Solutions for Better Health Care III, Brussels, Belgium, 2012/04/16. https://doi.org/10.1117/12.922698

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title = "Single-layer graphene based SPR biochips for tuberculosis bacillus detection",

abstract = "This paper is intended to demonstrate a facile and effective method to construct single layer graphene films onto the self-assembled monolayer (SAM) at Au electrodes based surface plasmon resonance (SPR) biochips integrated loop-mediated isothermal amplification (LAMP) for tuberculosis bacillus (TB) detection. It is a novel Au-SAM-graphene nanocomposites and taking advantages of the striking properties of both graphene and Au film, fundamental understanding in hybrid material manipulation and new electrochemical properties can be obtained. The sensitivity of TB detection in the LAMP-based assay for the amplification of the Insertion Sequence 6110 (IS6110) samples was determined by a single-layer graphene/Au thin film and compared with that of a conventional Au/Cr-based SPR chips. The results show that a graphene/Au SPR offers a potentially powerful assay, with a highly sensitive analysis, that may be applicable as an important tool for bio-marker detection.",

keywords = "Biosensor, Graphene, Insertion Sequence 6110 (IS6110), Loop-mediated isothermal amplification (LAMP), Self-assembled monolayer (SAM), Surface plasmon resonance (SPR), Tuberculosis bacillus (TB)",

author = "Chiu, \{Nan Fu\} and Huang, \{Teng Yi\} and Kuo, \{Chun Chuan\} and Lee, \{Wei Che\} and Hsieh, \{Min Hua\} and Lai, \{Hsin Chih\}",

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AU - Lee, Wei Che

AU - Hsieh, Min Hua

AU - Lai, Hsin Chih

PY - 2012

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N2 - This paper is intended to demonstrate a facile and effective method to construct single layer graphene films onto the self-assembled monolayer (SAM) at Au electrodes based surface plasmon resonance (SPR) biochips integrated loop-mediated isothermal amplification (LAMP) for tuberculosis bacillus (TB) detection. It is a novel Au-SAM-graphene nanocomposites and taking advantages of the striking properties of both graphene and Au film, fundamental understanding in hybrid material manipulation and new electrochemical properties can be obtained. The sensitivity of TB detection in the LAMP-based assay for the amplification of the Insertion Sequence 6110 (IS6110) samples was determined by a single-layer graphene/Au thin film and compared with that of a conventional Au/Cr-based SPR chips. The results show that a graphene/Au SPR offers a potentially powerful assay, with a highly sensitive analysis, that may be applicable as an important tool for bio-marker detection.

AB - This paper is intended to demonstrate a facile and effective method to construct single layer graphene films onto the self-assembled monolayer (SAM) at Au electrodes based surface plasmon resonance (SPR) biochips integrated loop-mediated isothermal amplification (LAMP) for tuberculosis bacillus (TB) detection. It is a novel Au-SAM-graphene nanocomposites and taking advantages of the striking properties of both graphene and Au film, fundamental understanding in hybrid material manipulation and new electrochemical properties can be obtained. The sensitivity of TB detection in the LAMP-based assay for the amplification of the Insertion Sequence 6110 (IS6110) samples was determined by a single-layer graphene/Au thin film and compared with that of a conventional Au/Cr-based SPR chips. The results show that a graphene/Au SPR offers a potentially powerful assay, with a highly sensitive analysis, that may be applicable as an important tool for bio-marker detection.

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KW - Graphene

KW - Insertion Sequence 6110 (IS6110)

KW - Loop-mediated isothermal amplification (LAMP)

KW - Self-assembled monolayer (SAM)

KW - Surface plasmon resonance (SPR)

KW - Tuberculosis bacillus (TB)

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ER -

Single-layer graphene based SPR biochips for tuberculosis bacillus detection

Abstract

Publication series

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UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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