Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip

J. H. Chien; D. S. Lee; W. P. Chou; P. Y. Wang; C. R. Yang; M. H. Wu; C. Y. Tsai; T. L. Chang; Y. W. Lee; Y. T. Cheng; P. H. Chen

doi:10.1109/NEMS.2006.334721

Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip

J. H. Chien, D. S. Lee, W. P. Chou, P. Y. Wang, C. R. Yang, M. H. Wu, C. Y. Tsai, T. L. Chang, Y. W. Lee, Y. T. Cheng, P. H. Chen

Department of Mechatronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

The current real-time PCR (polymerase chain reaction) platforms, which can detect and quantify several target DNA simultaneously, are equipped with discrete optics and detectors for different fluorescence wavelengths. However, the optical loss, due to the different lengths of the channels for several dyes, lowers the performance of fluorescence detection. Especially on the PCR platforms of lab-on-a-system, for the dispersion of the fluorescence in the micro fluidic channels, the received fluorescence is much lower than the emitted. To enhance the received intensity on the detection system is therefore a critical issue. The proposed fluorescence detection system, composing of an ultra-sensitive spectrometer, can provide continuous wavelength detection and can be employed for multiple DNA quantification and genotyping in a single reaction. For the tests to the genotyping ability, the melting temperatures of B type HBV and C type HBV can be distinguished by the difference of 1.1 C.The test results in this research show the same degree of sensitivity for DNA quantification and reproducibility within five intra assay samples as compared with a commercial one.

Original language	English
Title of host publication	Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
Pages	274-277
Number of pages	4
DOIs	https://doi.org/10.1109/NEMS.2006.334721
Publication status	Published - 2006
Event	1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS - Zhuhai, China Duration: 2006 Jan 18 → 2006 Jan 21

Publication series

Name	Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS

Other

Other	1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
Country/Territory	China
City	Zhuhai
Period	2006/01/18 → 2006/01/21

Keywords

HBV
HCV
Real-time PCR

ASJC Scopus subject areas

Biotechnology
Materials Science (miscellaneous)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/NEMS.2006.334721

Cite this

Chien, J. H., Lee, D. S., Chou, W. P., Wang, P. Y., Yang, C. R., Wu, M. H., Tsai, C. Y., Chang, T. L., Lee, Y. W., Cheng, Y. T., & Chen, P. H. (2006). Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip. In Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS (pp. 274-277). [4134951] (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS). https://doi.org/10.1109/NEMS.2006.334721

Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip. / Chien, J. H.; Lee, D. S.; Chou, W. P. et al.
Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS. 2006. p. 274-277 4134951 (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Chien, JH, Lee, DS, Chou, WP, Wang, PY, Yang, CR, Wu, MH, Tsai, CY, Chang, TL, Lee, YW, Cheng, YT & Chen, PH 2006, Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip. in Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS., 4134951, Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS, pp. 274-277, 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS, Zhuhai, China, 2006/01/18. https://doi.org/10.1109/NEMS.2006.334721

Chien JH, Lee DS, Chou WP, Wang PY, Yang CR, Wu MH et al. Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip. In Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS. 2006. p. 274-277. 4134951. (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS). doi: 10.1109/NEMS.2006.334721

Chien, J. H. ; Lee, D. S. ; Chou, W. P. et al. / Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip. Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS. 2006. pp. 274-277 (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS).

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title = "Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip",

abstract = "The current real-time PCR (polymerase chain reaction) platforms, which can detect and quantify several target DNA simultaneously, are equipped with discrete optics and detectors for different fluorescence wavelengths. However, the optical loss, due to the different lengths of the channels for several dyes, lowers the performance of fluorescence detection. Especially on the PCR platforms of lab-on-a-system, for the dispersion of the fluorescence in the micro fluidic channels, the received fluorescence is much lower than the emitted. To enhance the received intensity on the detection system is therefore a critical issue. The proposed fluorescence detection system, composing of an ultra-sensitive spectrometer, can provide continuous wavelength detection and can be employed for multiple DNA quantification and genotyping in a single reaction. For the tests to the genotyping ability, the melting temperatures of B type HBV and C type HBV can be distinguished by the difference of 1.1 C.The test results in this research show the same degree of sensitivity for DNA quantification and reproducibility within five intra assay samples as compared with a commercial one.",

keywords = "HBV, HCV, Real-time PCR",

author = "Chien, {J. H.} and Lee, {D. S.} and Chou, {W. P.} and Wang, {P. Y.} and Yang, {C. R.} and Wu, {M. H.} and Tsai, {C. Y.} and Chang, {T. L.} and Lee, {Y. W.} and Cheng, {Y. T.} and Chen, {P. H.}",

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AU - Chien, J. H.

AU - Lee, D. S.

AU - Chou, W. P.

AU - Wang, P. Y.

AU - Yang, C. R.

AU - Wu, M. H.

AU - Tsai, C. Y.

AU - Chang, T. L.

AU - Lee, Y. W.

AU - Cheng, Y. T.

AU - Chen, P. H.

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N2 - The current real-time PCR (polymerase chain reaction) platforms, which can detect and quantify several target DNA simultaneously, are equipped with discrete optics and detectors for different fluorescence wavelengths. However, the optical loss, due to the different lengths of the channels for several dyes, lowers the performance of fluorescence detection. Especially on the PCR platforms of lab-on-a-system, for the dispersion of the fluorescence in the micro fluidic channels, the received fluorescence is much lower than the emitted. To enhance the received intensity on the detection system is therefore a critical issue. The proposed fluorescence detection system, composing of an ultra-sensitive spectrometer, can provide continuous wavelength detection and can be employed for multiple DNA quantification and genotyping in a single reaction. For the tests to the genotyping ability, the melting temperatures of B type HBV and C type HBV can be distinguished by the difference of 1.1 C.The test results in this research show the same degree of sensitivity for DNA quantification and reproducibility within five intra assay samples as compared with a commercial one.

AB - The current real-time PCR (polymerase chain reaction) platforms, which can detect and quantify several target DNA simultaneously, are equipped with discrete optics and detectors for different fluorescence wavelengths. However, the optical loss, due to the different lengths of the channels for several dyes, lowers the performance of fluorescence detection. Especially on the PCR platforms of lab-on-a-system, for the dispersion of the fluorescence in the micro fluidic channels, the received fluorescence is much lower than the emitted. To enhance the received intensity on the detection system is therefore a critical issue. The proposed fluorescence detection system, composing of an ultra-sensitive spectrometer, can provide continuous wavelength detection and can be employed for multiple DNA quantification and genotyping in a single reaction. For the tests to the genotyping ability, the melting temperatures of B type HBV and C type HBV can be distinguished by the difference of 1.1 C.The test results in this research show the same degree of sensitivity for DNA quantification and reproducibility within five intra assay samples as compared with a commercial one.

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KW - HCV

KW - Real-time PCR

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Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip

Abstract

Publication series

Other

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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