Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip

J. H. Chien, D. S. Lee, W. P. Chou, P. Y. Wang, Chii-Rong Yang, M. H. Wu, C. Y. Tsai, Tien-Li Chang, Y. W. Lee, Y. T. Cheng, P. H. Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

The current real-time PCR (polymerase chain reaction) platforms, which can detect and quantify several target DNA simultaneously, are equipped with discrete optics and detectors for different fluorescence wavelengths. However, the optical loss, due to the different lengths of the channels for several dyes, lowers the performance of fluorescence detection. Especially on the PCR platforms of lab-on-a-system, for the dispersion of the fluorescence in the micro fluidic channels, the received fluorescence is much lower than the emitted. To enhance the received intensity on the detection system is therefore a critical issue. The proposed fluorescence detection system, composing of an ultra-sensitive spectrometer, can provide continuous wavelength detection and can be employed for multiple DNA quantification and genotyping in a single reaction. For the tests to the genotyping ability, the melting temperatures of B type HBV and C type HBV can be distinguished by the difference of 1.1 C.The test results in this research show the same degree of sensitivity for DNA quantification and reproducibility within five intra assay samples as compared with a commercial one.

Original languageEnglish
Title of host publicationProceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
Pages274-277
Number of pages4
DOIs
Publication statusPublished - 2006 Dec 1
Event1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS - Zhuhai, China
Duration: 2006 Jan 182006 Jan 21

Publication series

NameProceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS

Other

Other1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
CountryChina
CityZhuhai
Period06/1/1806/1/21

Fingerprint

Lab-on-a-chip
Polymerase chain reaction
Viruses
Hepatitis B virus
Hepacivirus
Real-Time Polymerase Chain Reaction
Fluorescence
DNA
Wavelength
Optical losses
Fluidics
Freezing
Melting point
Spectrometers
Assays
Optics
Coloring Agents
Dyes
Detectors
Polymerase Chain Reaction

Keywords

  • HBV
  • HCV
  • Real-time PCR

ASJC Scopus subject areas

  • Biotechnology
  • Materials Science (miscellaneous)

Cite this

Chien, J. H., Lee, D. S., Chou, W. P., Wang, P. Y., Yang, C-R., Wu, M. H., ... Chen, P. H. (2006). Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip. In Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS (pp. 274-277). [4134951] (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS). https://doi.org/10.1109/NEMS.2006.334721

Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip. / Chien, J. H.; Lee, D. S.; Chou, W. P.; Wang, P. Y.; Yang, Chii-Rong; Wu, M. H.; Tsai, C. Y.; Chang, Tien-Li; Lee, Y. W.; Cheng, Y. T.; Chen, P. H.

Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS. 2006. p. 274-277 4134951 (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chien, JH, Lee, DS, Chou, WP, Wang, PY, Yang, C-R, Wu, MH, Tsai, CY, Chang, T-L, Lee, YW, Cheng, YT & Chen, PH 2006, Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip. in Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS., 4134951, Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS, pp. 274-277, 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS, Zhuhai, China, 06/1/18. https://doi.org/10.1109/NEMS.2006.334721
Chien JH, Lee DS, Chou WP, Wang PY, Yang C-R, Wu MH et al. Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip. In Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS. 2006. p. 274-277. 4134951. (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS). https://doi.org/10.1109/NEMS.2006.334721
Chien, J. H. ; Lee, D. S. ; Chou, W. P. ; Wang, P. Y. ; Yang, Chii-Rong ; Wu, M. H. ; Tsai, C. Y. ; Chang, Tien-Li ; Lee, Y. W. ; Cheng, Y. T. ; Chen, P. H. / Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip. Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS. 2006. pp. 274-277 (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS).
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