In this paper, we report the enhanced performance of Graphene-Nafion-GOD based enzymatic biosensors to detection glucose used fast, simple, and easy to control fabrication method. These materials are prepared by mixing graphene, a Nafion perfluorinated resin, and glucose oxidase (GOD) in appropriate amounts. The graphene has a diameter of 8 μm. As a result, the sensitivity of lower GOD concentration is two times the higher concentration. It can be seen that the sensitivity was enhanced by graphene. The sensor was characterized electrochemically using cyclic voltammetry and amperometry in the presence of hydrogen peroxide and glucose.