Significance of the tissue kallikrein promoter and transforming growth factor-β1 polymorphisms with renal progression in children with vesicoureteral reflux

Guey Jen Lee-Chen, Kuo Pao Liu, Yi Chun Lai, Huei Shiuan Juang, Shu Yi Huang, Ching Yuang Lin

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background. Tissue kallikrein regulates blood circulation. Low urinary kallikrein excretion was associated with hypertension and renal disease in blacks. The polymorphic KLK1 promoter includes -130 GN coupled with multiple single base substitutions. The -130 G12 allele in the KLK1 promoter was associated with lower transcriptional activity and hypertensive end-stage renal disease (ESRD) in blacks. Transforming growth factor-β1 (TGF-β1) regulates matrix production, and induces fibrosis in a variety of tissues. High circulating TGF-β1 levels mediating renal fibrosis and loss of function in transgenic mice. The -509 T allele in the TGF-β1 promoter showed marginally higher transcriptional activity, and was associated with increased TGF-β1 production in humans. The aim of this study was to investigate whether the tissue KLK1 promoter and TGF-β1 polymorphism are involved in primary vesicoureteric reflux (VUR) with renal progression in children. Methods. Seventy-four primary VUR children were studied with regular annual follow-up for more than 18 years, all of them more than grade II (diagnosed by voiding cystourethroradiography). All of them were born before 1984. Patients were classified into two groups according to the renal function with progressive deterioration or not. Patients with baseline creatinine clearance (CCr) less than 25 mL/min were defined as having chronic renal insufficiency (CRI). The TGF-β1 -509 T-C polymorphism was analyzed by Bsu36I restriction fragment length polymorphism (RFLP)-polymerase chain reaction (PCR). In KLK1 promoter, the -130 GN length polymorphism and multiple single base substitutions were analyzed by electrophoresis of fluoresced PCR products in sequencing gels, single strand conformation polymorphism (SSCP), allele-specific PCR, and DNA sequencing. Patients' TGF-β1 and KLK1 promoter polymorphisms were evaluated for association with VUR susceptibility and progression in Taiwanese children. Annual echocardiography study was used to evaluate left ventricular mass index (LVMI). Results. Four alleles were identified in the complex KLK1 promoter: A (-130 G10), B (-130 G2CG7), H (-130 G11), and K (-130 G12). The polymorphic KLK1 promoter showed no association with VUR susceptibility. However, the frequency distribution of KLK1 promoter among VUR patients with or without CRI (A, 50.0% and 67.5%; B, 17.9% and 8.3%; H, 14.3% and 18.3%; K, 17.9% and 5.8%, respectively) was statistically different (P = 0.008). Significantly higher K allele frequency was present in primary VUR with CRI children, as it was in the renal survival curve study. A significant increase of LVMI was also found in the A allele group compared with the non-A allele group of KLK1 promoter gene at the age of 18 years old with renal progression. The TGF-β1 gene polymorphism was determined, and we found significant over-representation of the TT genotype in primary VUR patients with CRI compared with normal renal function (P = 0.0035). Conclusion. The K allele of KLK1 promoter and TT genotype of TGF-β1 may be a genetic KLK1 -130 GN and -128 G-C, and the susceptibility factor contributing to progressive renal deterioration in Taiwanese primary VUR children.

Original languageEnglish
Pages (from-to)1467-1472
Number of pages6
JournalKidney International
Volume65
Issue number4
DOIs
Publication statusPublished - 2004 Apr

Fingerprint

Tissue Kallikreins
Vesico-Ureteral Reflux
Transforming Growth Factors
Kidney
Alleles
Chronic Renal Insufficiency
Polymerase Chain Reaction
Fibrosis
Genotype
Renal Hypertension
Blood Circulation
DNA Sequence Analysis
Gene Frequency
Restriction Fragment Length Polymorphisms
Transgenic Mice
Genes
Chronic Kidney Failure
Echocardiography
Electrophoresis
Creatinine

Keywords

  • Chronic renal disease
  • Kallikrein
  • Left ventricular mass index (LVMI)
  • Polymorphisms
  • Transforming growth factor-β1
  • Vesicoureteral reflux

ASJC Scopus subject areas

  • Nephrology

Cite this

Significance of the tissue kallikrein promoter and transforming growth factor-β1 polymorphisms with renal progression in children with vesicoureteral reflux. / Lee-Chen, Guey Jen; Liu, Kuo Pao; Lai, Yi Chun; Juang, Huei Shiuan; Huang, Shu Yi; Lin, Ching Yuang.

In: Kidney International, Vol. 65, No. 4, 04.2004, p. 1467-1472.

Research output: Contribution to journalArticle

Lee-Chen, Guey Jen ; Liu, Kuo Pao ; Lai, Yi Chun ; Juang, Huei Shiuan ; Huang, Shu Yi ; Lin, Ching Yuang. / Significance of the tissue kallikrein promoter and transforming growth factor-β1 polymorphisms with renal progression in children with vesicoureteral reflux. In: Kidney International. 2004 ; Vol. 65, No. 4. pp. 1467-1472.
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abstract = "Background. Tissue kallikrein regulates blood circulation. Low urinary kallikrein excretion was associated with hypertension and renal disease in blacks. The polymorphic KLK1 promoter includes -130 GN coupled with multiple single base substitutions. The -130 G12 allele in the KLK1 promoter was associated with lower transcriptional activity and hypertensive end-stage renal disease (ESRD) in blacks. Transforming growth factor-β1 (TGF-β1) regulates matrix production, and induces fibrosis in a variety of tissues. High circulating TGF-β1 levels mediating renal fibrosis and loss of function in transgenic mice. The -509 T allele in the TGF-β1 promoter showed marginally higher transcriptional activity, and was associated with increased TGF-β1 production in humans. The aim of this study was to investigate whether the tissue KLK1 promoter and TGF-β1 polymorphism are involved in primary vesicoureteric reflux (VUR) with renal progression in children. Methods. Seventy-four primary VUR children were studied with regular annual follow-up for more than 18 years, all of them more than grade II (diagnosed by voiding cystourethroradiography). All of them were born before 1984. Patients were classified into two groups according to the renal function with progressive deterioration or not. Patients with baseline creatinine clearance (CCr) less than 25 mL/min were defined as having chronic renal insufficiency (CRI). The TGF-β1 -509 T-C polymorphism was analyzed by Bsu36I restriction fragment length polymorphism (RFLP)-polymerase chain reaction (PCR). In KLK1 promoter, the -130 GN length polymorphism and multiple single base substitutions were analyzed by electrophoresis of fluoresced PCR products in sequencing gels, single strand conformation polymorphism (SSCP), allele-specific PCR, and DNA sequencing. Patients' TGF-β1 and KLK1 promoter polymorphisms were evaluated for association with VUR susceptibility and progression in Taiwanese children. Annual echocardiography study was used to evaluate left ventricular mass index (LVMI). Results. Four alleles were identified in the complex KLK1 promoter: A (-130 G10), B (-130 G2CG7), H (-130 G11), and K (-130 G12). The polymorphic KLK1 promoter showed no association with VUR susceptibility. However, the frequency distribution of KLK1 promoter among VUR patients with or without CRI (A, 50.0{\%} and 67.5{\%}; B, 17.9{\%} and 8.3{\%}; H, 14.3{\%} and 18.3{\%}; K, 17.9{\%} and 5.8{\%}, respectively) was statistically different (P = 0.008). Significantly higher K allele frequency was present in primary VUR with CRI children, as it was in the renal survival curve study. A significant increase of LVMI was also found in the A allele group compared with the non-A allele group of KLK1 promoter gene at the age of 18 years old with renal progression. The TGF-β1 gene polymorphism was determined, and we found significant over-representation of the TT genotype in primary VUR patients with CRI compared with normal renal function (P = 0.0035). Conclusion. The K allele of KLK1 promoter and TT genotype of TGF-β1 may be a genetic KLK1 -130 GN and -128 G-C, and the susceptibility factor contributing to progressive renal deterioration in Taiwanese primary VUR children.",
keywords = "Chronic renal disease, Kallikrein, Left ventricular mass index (LVMI), Polymorphisms, Transforming growth factor-β1, Vesicoureteral reflux",
author = "Lee-Chen, {Guey Jen} and Liu, {Kuo Pao} and Lai, {Yi Chun} and Juang, {Huei Shiuan} and Huang, {Shu Yi} and Lin, {Ching Yuang}",
year = "2004",
month = "4",
doi = "10.1111/j.1523-1755.2004.00526.x",
language = "English",
volume = "65",
pages = "1467--1472",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
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TY - JOUR

T1 - Significance of the tissue kallikrein promoter and transforming growth factor-β1 polymorphisms with renal progression in children with vesicoureteral reflux

AU - Lee-Chen, Guey Jen

AU - Liu, Kuo Pao

AU - Lai, Yi Chun

AU - Juang, Huei Shiuan

AU - Huang, Shu Yi

AU - Lin, Ching Yuang

PY - 2004/4

Y1 - 2004/4

N2 - Background. Tissue kallikrein regulates blood circulation. Low urinary kallikrein excretion was associated with hypertension and renal disease in blacks. The polymorphic KLK1 promoter includes -130 GN coupled with multiple single base substitutions. The -130 G12 allele in the KLK1 promoter was associated with lower transcriptional activity and hypertensive end-stage renal disease (ESRD) in blacks. Transforming growth factor-β1 (TGF-β1) regulates matrix production, and induces fibrosis in a variety of tissues. High circulating TGF-β1 levels mediating renal fibrosis and loss of function in transgenic mice. The -509 T allele in the TGF-β1 promoter showed marginally higher transcriptional activity, and was associated with increased TGF-β1 production in humans. The aim of this study was to investigate whether the tissue KLK1 promoter and TGF-β1 polymorphism are involved in primary vesicoureteric reflux (VUR) with renal progression in children. Methods. Seventy-four primary VUR children were studied with regular annual follow-up for more than 18 years, all of them more than grade II (diagnosed by voiding cystourethroradiography). All of them were born before 1984. Patients were classified into two groups according to the renal function with progressive deterioration or not. Patients with baseline creatinine clearance (CCr) less than 25 mL/min were defined as having chronic renal insufficiency (CRI). The TGF-β1 -509 T-C polymorphism was analyzed by Bsu36I restriction fragment length polymorphism (RFLP)-polymerase chain reaction (PCR). In KLK1 promoter, the -130 GN length polymorphism and multiple single base substitutions were analyzed by electrophoresis of fluoresced PCR products in sequencing gels, single strand conformation polymorphism (SSCP), allele-specific PCR, and DNA sequencing. Patients' TGF-β1 and KLK1 promoter polymorphisms were evaluated for association with VUR susceptibility and progression in Taiwanese children. Annual echocardiography study was used to evaluate left ventricular mass index (LVMI). Results. Four alleles were identified in the complex KLK1 promoter: A (-130 G10), B (-130 G2CG7), H (-130 G11), and K (-130 G12). The polymorphic KLK1 promoter showed no association with VUR susceptibility. However, the frequency distribution of KLK1 promoter among VUR patients with or without CRI (A, 50.0% and 67.5%; B, 17.9% and 8.3%; H, 14.3% and 18.3%; K, 17.9% and 5.8%, respectively) was statistically different (P = 0.008). Significantly higher K allele frequency was present in primary VUR with CRI children, as it was in the renal survival curve study. A significant increase of LVMI was also found in the A allele group compared with the non-A allele group of KLK1 promoter gene at the age of 18 years old with renal progression. The TGF-β1 gene polymorphism was determined, and we found significant over-representation of the TT genotype in primary VUR patients with CRI compared with normal renal function (P = 0.0035). Conclusion. The K allele of KLK1 promoter and TT genotype of TGF-β1 may be a genetic KLK1 -130 GN and -128 G-C, and the susceptibility factor contributing to progressive renal deterioration in Taiwanese primary VUR children.

AB - Background. Tissue kallikrein regulates blood circulation. Low urinary kallikrein excretion was associated with hypertension and renal disease in blacks. The polymorphic KLK1 promoter includes -130 GN coupled with multiple single base substitutions. The -130 G12 allele in the KLK1 promoter was associated with lower transcriptional activity and hypertensive end-stage renal disease (ESRD) in blacks. Transforming growth factor-β1 (TGF-β1) regulates matrix production, and induces fibrosis in a variety of tissues. High circulating TGF-β1 levels mediating renal fibrosis and loss of function in transgenic mice. The -509 T allele in the TGF-β1 promoter showed marginally higher transcriptional activity, and was associated with increased TGF-β1 production in humans. The aim of this study was to investigate whether the tissue KLK1 promoter and TGF-β1 polymorphism are involved in primary vesicoureteric reflux (VUR) with renal progression in children. Methods. Seventy-four primary VUR children were studied with regular annual follow-up for more than 18 years, all of them more than grade II (diagnosed by voiding cystourethroradiography). All of them were born before 1984. Patients were classified into two groups according to the renal function with progressive deterioration or not. Patients with baseline creatinine clearance (CCr) less than 25 mL/min were defined as having chronic renal insufficiency (CRI). The TGF-β1 -509 T-C polymorphism was analyzed by Bsu36I restriction fragment length polymorphism (RFLP)-polymerase chain reaction (PCR). In KLK1 promoter, the -130 GN length polymorphism and multiple single base substitutions were analyzed by electrophoresis of fluoresced PCR products in sequencing gels, single strand conformation polymorphism (SSCP), allele-specific PCR, and DNA sequencing. Patients' TGF-β1 and KLK1 promoter polymorphisms were evaluated for association with VUR susceptibility and progression in Taiwanese children. Annual echocardiography study was used to evaluate left ventricular mass index (LVMI). Results. Four alleles were identified in the complex KLK1 promoter: A (-130 G10), B (-130 G2CG7), H (-130 G11), and K (-130 G12). The polymorphic KLK1 promoter showed no association with VUR susceptibility. However, the frequency distribution of KLK1 promoter among VUR patients with or without CRI (A, 50.0% and 67.5%; B, 17.9% and 8.3%; H, 14.3% and 18.3%; K, 17.9% and 5.8%, respectively) was statistically different (P = 0.008). Significantly higher K allele frequency was present in primary VUR with CRI children, as it was in the renal survival curve study. A significant increase of LVMI was also found in the A allele group compared with the non-A allele group of KLK1 promoter gene at the age of 18 years old with renal progression. The TGF-β1 gene polymorphism was determined, and we found significant over-representation of the TT genotype in primary VUR patients with CRI compared with normal renal function (P = 0.0035). Conclusion. The K allele of KLK1 promoter and TT genotype of TGF-β1 may be a genetic KLK1 -130 GN and -128 G-C, and the susceptibility factor contributing to progressive renal deterioration in Taiwanese primary VUR children.

KW - Chronic renal disease

KW - Kallikrein

KW - Left ventricular mass index (LVMI)

KW - Polymorphisms

KW - Transforming growth factor-β1

KW - Vesicoureteral reflux

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