TY - JOUR
T1 - Separation of nine iridoids by capillary electrophoresis and high performance liquid chromatography
AU - Wu, Hsin Kai
AU - Chuang, Wu Chang
AU - Sheu, Shuenn Jyi
N1 - Funding Information:
Financial support from the National Science Council, Taiwan, is gratefully acknowledged (NSC 86-2113-M-003-010).
PY - 1998/4/17
Y1 - 1998/4/17
N2 - A capillary zone electrophoretic (CZE) method and a high-performance liquid chromatographic (HPLC) method were developed for the separation of the nine iridoids, gardenoside, geniposide, geniposidic acid, shanzhiside, loganin, loganic acid, aucubin, harpagoside and catapol. Detection at 210 and 230 nm with a 2,6-di-O-methyl-β-cyclodextrin and sodium borate buffer as carrier or with a linear gradient elution system using acetonitrile and potassium dihydrogenphosphate solution as eluent was found to be the most suitable approach for this separation. The CZE analysis time (32 min) was shorter than that of HPLC (45 min), but the CE method can separate only eight of the nine compounds. The pH, buffer concentration and organic composition of the mobile phase were studied for their effects on the separability of the compounds.
AB - A capillary zone electrophoretic (CZE) method and a high-performance liquid chromatographic (HPLC) method were developed for the separation of the nine iridoids, gardenoside, geniposide, geniposidic acid, shanzhiside, loganin, loganic acid, aucubin, harpagoside and catapol. Detection at 210 and 230 nm with a 2,6-di-O-methyl-β-cyclodextrin and sodium borate buffer as carrier or with a linear gradient elution system using acetonitrile and potassium dihydrogenphosphate solution as eluent was found to be the most suitable approach for this separation. The CZE analysis time (32 min) was shorter than that of HPLC (45 min), but the CE method can separate only eight of the nine compounds. The pH, buffer concentration and organic composition of the mobile phase were studied for their effects on the separability of the compounds.
KW - Buffer composition
KW - Iridoids
KW - Mobile phase composition
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U2 - 10.1016/S0021-9673(97)01227-2
DO - 10.1016/S0021-9673(97)01227-2
M3 - Article
AN - SCOPUS:0031965680
SN - 0021-9673
VL - 803
SP - 179
EP - 187
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -