Separation of nine iridoids by capillary electrophoresis and high performance liquid chromatography

Hsin Kai Wu*, Wu Chang Chuang, Shuenn Jyi Sheu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)


A capillary zone electrophoretic (CZE) method and a high-performance liquid chromatographic (HPLC) method were developed for the separation of the nine iridoids, gardenoside, geniposide, geniposidic acid, shanzhiside, loganin, loganic acid, aucubin, harpagoside and catapol. Detection at 210 and 230 nm with a 2,6-di-O-methyl-β-cyclodextrin and sodium borate buffer as carrier or with a linear gradient elution system using acetonitrile and potassium dihydrogenphosphate solution as eluent was found to be the most suitable approach for this separation. The CZE analysis time (32 min) was shorter than that of HPLC (45 min), but the CE method can separate only eight of the nine compounds. The pH, buffer concentration and organic composition of the mobile phase were studied for their effects on the separability of the compounds.

Original languageEnglish
Pages (from-to)179-187
Number of pages9
JournalJournal of Chromatography A
Issue number1-2
Publication statusPublished - 1998 Apr 17


  • Buffer composition
  • Iridoids
  • Mobile phase composition

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


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