Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles

Ying Feng Chang; Bao Yu Hsieh; Hsiang Yin Chen; Ming Yaw Ng; Wei Chih Liu; Yung Chang Chung; Hsieh Ting Wu; Chien Chou

doi:10.1117/12.699113

Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles

Ying Feng Chang^*, Bao Yu Hsieh, Hsiang Yin Chen, Ming Yaw Ng, Wei Chih Liu, Yung Chang Chung, Hsieh Ting Wu, Chien Chou

^*Corresponding author for this work

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

We proposed and developed a novel fiber-optic biosensor based on localized surface plasmon coupled emission (LSPCE) which consists of sandwich format of 〈antibody/antigen/Cy5-antibody-GNP〉 immuno-complex. It is immobilized on the surface of optical fiber where 〈Cy5-antibody-GNP〉 is a fluorescence probe produced by mixing Cy5 labeled antibody and protein A conjugated gold nanoparticles (Au-PA). The fluorophores are excited by localized surface plasmon (LSP) on gold nanoparticle (GNP) surface where the evanescent field is applied near the core surface of unclad optical fiber. Meanwhile, the fluorescence signal is detected by a photomultiplier tube being set beside the unclad optical fiber with high collection efficiency. In the experiment, this novel LSPCE biosensor demonstrates the minimum detectable concentration of mouse immunoglobulin G (IgG) at 1pg/ml (7fM) in the biomolecular interaction with anti-mouse IgG. From the experimental result, it verifies that LSPCE biosensor is a very high sensitive biosensor which is capable of measuring biomolecular interaction at very low concentration.

Original language	English
Title of host publication	Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
DOIs	https://doi.org/10.1117/12.699113
Publication status	Published - 2007
Event	Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV - San Jose, CA, United States Duration: 2007 Jan 23 → 2007 Jan 24

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6447
ISSN (Print)	1605-7422

Conference

Conference	Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
Country/Territory	United States
City	San Jose, CA
Period	2007/01/23 → 2007/01/24

Keywords

Fiber-optic biosensor
Gold nanoparticles
Localized surface plasmon

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.699113

Cite this

Chang, Y. F., Hsieh, B. Y., Chen, H. Y., Ng, M. Y., Liu, W. C., Chung, Y. C., Wu, H. T., & Chou, C. (2007). Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles. In Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV Article 64470R (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6447). https://doi.org/10.1117/12.699113

Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles. / Chang, Ying Feng; Hsieh, Bao Yu; Chen, Hsiang Yin et al.
Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV. 2007. 64470R (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6447).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Chang, YF, Hsieh, BY, Chen, HY, Ng, MY, Liu, WC, Chung, YC, Wu, HT & Chou, C 2007, Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles. in Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV., 64470R, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6447, Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV, San Jose, CA, United States, 2007/01/23. https://doi.org/10.1117/12.699113

Chang YF, Hsieh BY, Chen HY, Ng MY, Liu WC, Chung YC et al. Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles. In Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV. 2007. 64470R. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.699113

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title = "Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles",

abstract = "We proposed and developed a novel fiber-optic biosensor based on localized surface plasmon coupled emission (LSPCE) which consists of sandwich format of 〈antibody/antigen/Cy5-antibody-GNP〉 immuno-complex. It is immobilized on the surface of optical fiber where 〈Cy5-antibody-GNP〉 is a fluorescence probe produced by mixing Cy5 labeled antibody and protein A conjugated gold nanoparticles (Au-PA). The fluorophores are excited by localized surface plasmon (LSP) on gold nanoparticle (GNP) surface where the evanescent field is applied near the core surface of unclad optical fiber. Meanwhile, the fluorescence signal is detected by a photomultiplier tube being set beside the unclad optical fiber with high collection efficiency. In the experiment, this novel LSPCE biosensor demonstrates the minimum detectable concentration of mouse immunoglobulin G (IgG) at 1pg/ml (7fM) in the biomolecular interaction with anti-mouse IgG. From the experimental result, it verifies that LSPCE biosensor is a very high sensitive biosensor which is capable of measuring biomolecular interaction at very low concentration.",

keywords = "Fiber-optic biosensor, Gold nanoparticles, Localized surface plasmon",

author = "Chang, {Ying Feng} and Hsieh, {Bao Yu} and Chen, {Hsiang Yin} and Ng, {Ming Yaw} and Liu, {Wei Chih} and Chung, {Yung Chang} and Wu, {Hsieh Ting} and Chien Chou",

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AU - Chang, Ying Feng

AU - Hsieh, Bao Yu

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AU - Liu, Wei Chih

AU - Chung, Yung Chang

AU - Wu, Hsieh Ting

AU - Chou, Chien

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AB - We proposed and developed a novel fiber-optic biosensor based on localized surface plasmon coupled emission (LSPCE) which consists of sandwich format of 〈antibody/antigen/Cy5-antibody-GNP〉 immuno-complex. It is immobilized on the surface of optical fiber where 〈Cy5-antibody-GNP〉 is a fluorescence probe produced by mixing Cy5 labeled antibody and protein A conjugated gold nanoparticles (Au-PA). The fluorophores are excited by localized surface plasmon (LSP) on gold nanoparticle (GNP) surface where the evanescent field is applied near the core surface of unclad optical fiber. Meanwhile, the fluorescence signal is detected by a photomultiplier tube being set beside the unclad optical fiber with high collection efficiency. In the experiment, this novel LSPCE biosensor demonstrates the minimum detectable concentration of mouse immunoglobulin G (IgG) at 1pg/ml (7fM) in the biomolecular interaction with anti-mouse IgG. From the experimental result, it verifies that LSPCE biosensor is a very high sensitive biosensor which is capable of measuring biomolecular interaction at very low concentration.

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ER -

Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles

Abstract

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Keywords

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