Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles

Ying Feng Chang, Bao Yu Hsieh, Hsiang Yin Chen, Ming Yaw Ng, Wei-Chih Liu, Yung Chang Chung, Hsieh Ting Wu, Chien Chou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We proposed and developed a novel fiber-optic biosensor based on localized surface plasmon coupled emission (LSPCE) which consists of sandwich format of 〈antibody/antigen/Cy5-antibody-GNP〉 immuno-complex. It is immobilized on the surface of optical fiber where 〈Cy5-antibody-GNP〉 is a fluorescence probe produced by mixing Cy5 labeled antibody and protein A conjugated gold nanoparticles (Au-PA). The fluorophores are excited by localized surface plasmon (LSP) on gold nanoparticle (GNP) surface where the evanescent field is applied near the core surface of unclad optical fiber. Meanwhile, the fluorescence signal is detected by a photomultiplier tube being set beside the unclad optical fiber with high collection efficiency. In the experiment, this novel LSPCE biosensor demonstrates the minimum detectable concentration of mouse immunoglobulin G (IgG) at 1pg/ml (7fM) in the biomolecular interaction with anti-mouse IgG. From the experimental result, it verifies that LSPCE biosensor is a very high sensitive biosensor which is capable of measuring biomolecular interaction at very low concentration.

Original languageEnglish
Title of host publicationNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
Volume6447
DOIs
Publication statusPublished - 2007 Apr 30
EventNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV - San Jose, CA, United States
Duration: 2007 Jan 232007 Jan 24

Other

OtherNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
CountryUnited States
CitySan Jose, CA
Period07/1/2307/1/24

Fingerprint

Biosensing Techniques
Optical Fibers
bioinstrumentation
Biosensors
Gold
Nanoparticles
Fiber optics
fiber optics
gold
nanoparticles
Antibodies
augmentation
antibodies
sensitivity
Immunoglobulin G
Fluorescence
Optical fibers
optical fibers
Staphylococcal Protein A
mice

Keywords

  • Fiber-optic biosensor
  • Gold nanoparticles
  • Localized surface plasmon

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Chang, Y. F., Hsieh, B. Y., Chen, H. Y., Ng, M. Y., Liu, W-C., Chung, Y. C., ... Chou, C. (2007). Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles. In Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV (Vol. 6447). [64470R] https://doi.org/10.1117/12.699113

Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles. / Chang, Ying Feng; Hsieh, Bao Yu; Chen, Hsiang Yin; Ng, Ming Yaw; Liu, Wei-Chih; Chung, Yung Chang; Wu, Hsieh Ting; Chou, Chien.

Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV. Vol. 6447 2007. 64470R.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chang, YF, Hsieh, BY, Chen, HY, Ng, MY, Liu, W-C, Chung, YC, Wu, HT & Chou, C 2007, Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles. in Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV. vol. 6447, 64470R, Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV, San Jose, CA, United States, 07/1/23. https://doi.org/10.1117/12.699113
Chang YF, Hsieh BY, Chen HY, Ng MY, Liu W-C, Chung YC et al. Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles. In Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV. Vol. 6447. 2007. 64470R https://doi.org/10.1117/12.699113
Chang, Ying Feng ; Hsieh, Bao Yu ; Chen, Hsiang Yin ; Ng, Ming Yaw ; Liu, Wei-Chih ; Chung, Yung Chang ; Wu, Hsieh Ting ; Chou, Chien. / Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles. Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV. Vol. 6447 2007.
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