Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles

Ying Feng Chang, Bao Yu Hsieh, Hsiang Yin Chen, Ming Yaw Ng, Wei-Chih Liu, Yung Chang Chung, Hsieh Ting Wu, Chien Chou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We proposed and developed a novel fiber-optic biosensor based on localized surface plasmon coupled emission (LSPCE) which consists of sandwich format of 〈antibody/antigen/Cy5-antibody-GNP〉 immuno-complex. It is immobilized on the surface of optical fiber where 〈Cy5-antibody-GNP〉 is a fluorescence probe produced by mixing Cy5 labeled antibody and protein A conjugated gold nanoparticles (Au-PA). The fluorophores are excited by localized surface plasmon (LSP) on gold nanoparticle (GNP) surface where the evanescent field is applied near the core surface of unclad optical fiber. Meanwhile, the fluorescence signal is detected by a photomultiplier tube being set beside the unclad optical fiber with high collection efficiency. In the experiment, this novel LSPCE biosensor demonstrates the minimum detectable concentration of mouse immunoglobulin G (IgG) at 1pg/ml (7fM) in the biomolecular interaction with anti-mouse IgG. From the experimental result, it verifies that LSPCE biosensor is a very high sensitive biosensor which is capable of measuring biomolecular interaction at very low concentration.

Original languageEnglish
Title of host publicationNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
Volume6447
DOIs
Publication statusPublished - 2007 Apr 30
EventNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV - San Jose, CA, United States
Duration: 2007 Jan 232007 Jan 24

Other

OtherNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
CountryUnited States
CitySan Jose, CA
Period07/1/2307/1/24

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Keywords

  • Fiber-optic biosensor
  • Gold nanoparticles
  • Localized surface plasmon

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Chang, Y. F., Hsieh, B. Y., Chen, H. Y., Ng, M. Y., Liu, W-C., Chung, Y. C., Wu, H. T., & Chou, C. (2007). Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles. In Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV (Vol. 6447). [64470R] https://doi.org/10.1117/12.699113