TY - JOUR
T1 - Sensitivity and kinetic analysis of graphene oxide-based surface plasmon resonance biosensors
AU - Chiu, Nan Fu
AU - Huang, Teng Yi
N1 - Funding Information:
This work was supported in part by the National Science Council of the Republic of China (Taiwan) under Grant NSC 99-2218-E-003-002-MY3, NSC 102-2221-E-003-021 and Grant NSC 100-2325-B-182-007 and by the Emerging Research and Development Projects of National Taiwan Normal University under Grant NTNU100-D-01.
PY - 2014/7/5
Y1 - 2014/7/5
N2 - In this study, we demonstrate the fabrication of graphene oxide sheet (GOS)-based surface plasmon resonance (SPR) sensors for protein immobilization detection. GOS was used as the medium owing to its high covalent binding affinity for proteins. We were able to monitor the binding phenomenon in real time using the SPR-based technique. Thus, this GOS sensor can detect bovine albumin serum (BSA) directly in GOS films and exhibited up to a 4.3-fold higher angle shift than that of the conventional Au/Cr-based sensor in 10 ng/ml of BSA. The results showed that the SPR biosensor formed using a GOS solution of 2 mg/ml has optimal sensitive and had a BSA detection limit as low as 100 pg/ml. The sensitivity of the slope ratio of the GOS vs. conventional Au substrate is 2.4:1. Moreover, GOS is a label-free detection without additional amplification steps to enhance the sensitivity. In addition, the average value of the affinity binding constant KA for the GOS-based sensor with respect to BSA was 80.8 × 106 M-1. These results suggest that GOS-based SPR biosensors have potential for use in high-sensitivity protein immobilization detection.
AB - In this study, we demonstrate the fabrication of graphene oxide sheet (GOS)-based surface plasmon resonance (SPR) sensors for protein immobilization detection. GOS was used as the medium owing to its high covalent binding affinity for proteins. We were able to monitor the binding phenomenon in real time using the SPR-based technique. Thus, this GOS sensor can detect bovine albumin serum (BSA) directly in GOS films and exhibited up to a 4.3-fold higher angle shift than that of the conventional Au/Cr-based sensor in 10 ng/ml of BSA. The results showed that the SPR biosensor formed using a GOS solution of 2 mg/ml has optimal sensitive and had a BSA detection limit as low as 100 pg/ml. The sensitivity of the slope ratio of the GOS vs. conventional Au substrate is 2.4:1. Moreover, GOS is a label-free detection without additional amplification steps to enhance the sensitivity. In addition, the average value of the affinity binding constant KA for the GOS-based sensor with respect to BSA was 80.8 × 106 M-1. These results suggest that GOS-based SPR biosensors have potential for use in high-sensitivity protein immobilization detection.
KW - Bovine albumin serum
KW - Graphene oxide sheet
KW - Surface plasmon resonance
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U2 - 10.1016/j.snb.2014.02.033
DO - 10.1016/j.snb.2014.02.033
M3 - Article
AN - SCOPUS:84896541223
SN - 0925-4005
VL - 197
SP - 35
EP - 42
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -