Sensitivity and kinetic analysis of graphene oxide-based surface plasmon resonance biosensors

Nan-Fu Chiu, Teng Yi Huang

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

In this study, we demonstrate the fabrication of graphene oxide sheet (GOS)-based surface plasmon resonance (SPR) sensors for protein immobilization detection. GOS was used as the medium owing to its high covalent binding affinity for proteins. We were able to monitor the binding phenomenon in real time using the SPR-based technique. Thus, this GOS sensor can detect bovine albumin serum (BSA) directly in GOS films and exhibited up to a 4.3-fold higher angle shift than that of the conventional Au/Cr-based sensor in 10 ng/ml of BSA. The results showed that the SPR biosensor formed using a GOS solution of 2 mg/ml has optimal sensitive and had a BSA detection limit as low as 100 pg/ml. The sensitivity of the slope ratio of the GOS vs. conventional Au substrate is 2.4:1. Moreover, GOS is a label-free detection without additional amplification steps to enhance the sensitivity. In addition, the average value of the affinity binding constant KA for the GOS-based sensor with respect to BSA was 80.8 × 106 M-1. These results suggest that GOS-based SPR biosensors have potential for use in high-sensitivity protein immobilization detection.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume197
DOIs
Publication statusPublished - 2014 Jul 5

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Surface plasmon resonance
sensitivity analysis
bioinstrumentation
surface plasmon resonance
Biosensors
Oxides
Graphene
graphene
Kinetics
oxides
kinetics
sensors
Sensors
proteins
Proteins
immobilization
affinity
sensitivity
Bovine Serum Albumin

Keywords

  • Bovine albumin serum
  • Graphene oxide sheet
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Sensitivity and kinetic analysis of graphene oxide-based surface plasmon resonance biosensors. / Chiu, Nan-Fu; Huang, Teng Yi.

In: Sensors and Actuators, B: Chemical, Vol. 197, 05.07.2014, p. 35-42.

Research output: Contribution to journalArticle

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