Selective binding of mannose-encapsulated gold nanoparticles to type 1 pili in Escherichia coli

Chun Cheng Lin, Yi Chun Yeh, Chan Yi Yang, Chan Long Chen, Gee Fong Chen, Chia Chun Chen, Yi Chun Wu

Research output: Contribution to journalArticle

249 Citations (Scopus)

Abstract

The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.

Original languageEnglish
Pages (from-to)3508-3509
Number of pages2
JournalJournal of the American Chemical Society
Volume124
Issue number14
DOIs
Publication statusPublished - 2002 Apr 10

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Mannose
Gold
Nanoparticles
Escherichia coli
Biological systems
Carbohydrates
Labels
Assays
Bacterial Fimbriae
Agglomeration
Affinity Labels
Ligands
Transmission electron microscopy
Ions
Transmission Electron Microscopy

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Selective binding of mannose-encapsulated gold nanoparticles to type 1 pili in Escherichia coli. / Lin, Chun Cheng; Yeh, Yi Chun; Yang, Chan Yi; Chen, Chan Long; Chen, Gee Fong; Chen, Chia Chun; Wu, Yi Chun.

In: Journal of the American Chemical Society, Vol. 124, No. 14, 10.04.2002, p. 3508-3509.

Research output: Contribution to journalArticle

Lin, Chun Cheng ; Yeh, Yi Chun ; Yang, Chan Yi ; Chen, Chan Long ; Chen, Gee Fong ; Chen, Chia Chun ; Wu, Yi Chun. / Selective binding of mannose-encapsulated gold nanoparticles to type 1 pili in Escherichia coli. In: Journal of the American Chemical Society. 2002 ; Vol. 124, No. 14. pp. 3508-3509.
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