Selective binding of mannose-encapsulated gold nanoparticles to type 1 pili in Escherichia coli

Chun Cheng Lin, Yi Chun Yeh, Chan Yi Yang, Chan Long Chen, Gee Fong Chen, Chia Chun Chen, Yi Chun Wu

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253 Citations (Scopus)


The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.

Original languageEnglish
Pages (from-to)3508-3509
Number of pages2
JournalJournal of the American Chemical Society
Issue number14
Publication statusPublished - 2002 Apr 10


ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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