Screening transferable microsatellite markers across genus Phalaenopsis (Orchidaceae)

Ya Zhu Ko, Huei Chuan Shih, Chi Chu Tsai, Hsing Hua Ho, Pei-Chun Liao, Yu Chung Chiang

Research output: Contribution to journalArticle

Abstract

Background: Molecular identification based on microsatellite loci is an important technology to improve the commercial breeding of the moth orchid. There are more than 30,000 cultivars have been enrolled at the Royal Horticultural Society (RHS). In this study, genomic microsatellite primer sets were developed from Phalaenopsis aphrodite subsp. formosana to further examine the transferability of across 21 Phalaenopsis species. Methods and results: Twenty-eight polymorphic microsatellite markers were obtained using the magnetic bead enrichment method, with high transferability of the 21 species of the genus Phalaenopsis, especially in the subgenus Phalaenopsis. The 28 newly developed polymorphic microsatellite markers with high polymorphism information content values. The best and second fit grouping (K) are inferred as two and four by the ΔK evaluation in the assignment test. This result indicates that these microsatellite markers are discernible to subgenus Phalaenopsis. Conclusions: Our results indicate that these new microsatellite markers are useful for delimiting species within genus Phalaenopsis. As expected, the genetic relationships between species of subgenus Phalaenopsis can be well distinguished based on the assignment test. These molecular markers could apply to assess the paternity of Phalaenopsis as well as investigating hybridization among species of genus Phalaenopsis.

Original languageEnglish
Article number48
JournalBotanical Studies
Volume58
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Phalaenopsis
Orchidaceae
microsatellite repeats
screening
paternity
genetic relationships
hybridization
testing
genetic polymorphism
genomics

Keywords

  • Microsatellites
  • Phalaenopsis
  • Polymorphism
  • Transferability

ASJC Scopus subject areas

  • Plant Science

Cite this

Screening transferable microsatellite markers across genus Phalaenopsis (Orchidaceae). / Ko, Ya Zhu; Shih, Huei Chuan; Tsai, Chi Chu; Ho, Hsing Hua; Liao, Pei-Chun; Chiang, Yu Chung.

In: Botanical Studies, Vol. 58, No. 1, 48, 01.12.2017.

Research output: Contribution to journalArticle

Ko, Ya Zhu ; Shih, Huei Chuan ; Tsai, Chi Chu ; Ho, Hsing Hua ; Liao, Pei-Chun ; Chiang, Yu Chung. / Screening transferable microsatellite markers across genus Phalaenopsis (Orchidaceae). In: Botanical Studies. 2017 ; Vol. 58, No. 1.
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