Scavenging effects of lotus seed extracts on reactive nitrogen species

Gow Chin Yen; Pin Der Duh; Hui Ju Su; Chi Tai Yeh; Chi Hao Wu

doi:10.1016/j.foodchem.2004.11.052

Scavenging effects of lotus seed extracts on reactive nitrogen species

Gow Chin Yen^*, Pin Der Duh, Hui Ju Su, Chi Tai Yeh, Chi Hao Wu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

The inhibitory effect of lotus (Nelumbo nicifera) seed extracts extracted with water (LSWE), ethyl acetate (LSEAE) and hexane (LSHE) on reactive nitrogen species, induced by DNA damage in macrophage RAW 264.7 cells, was investigated. The results showed that all extracts could inhibit nitric oxide accumulation in LPS-activated RAW 264.7 cells. The extracts in the range of 0.01-0.2 mg/ml showed a dose-dependent inhibitory effect on the accumulation of nitric oxide upon decomposition of sodium nitroprusside (SNP). The potency of inhibitory activity was in the order: LSEAE > LSWE > LSHE. The results of the comet assay indicated that the three extracts could inhibit DNA damage in macrophage RAW 264.7 cells induced by SNP. In addition, the three samples, at 0.2 mg/ml, showed 63%, 59%, and 38% inhibition of DNA damage in macrophage RAW 264.7 cells induced by peroxynitrite, respectively. All extracts tested were found to be potent peroxynitrite scavengers, capable of preventing the nitration of tyrosine. The data obtained suggest that lotus seed extracts might act as chemopreventers through reduction of excess amounts of nitric oxide.

Original language	English
Pages (from-to)	596-602
Number of pages	7
Journal	Food Chemistry
Volume	94
Issue number	4
DOIs	https://doi.org/10.1016/j.foodchem.2004.11.052
Publication status	Published - 2006 Mar
Externally published	Yes

Keywords

Lotus seed
Macrophage RAW 264.7
Nitric oxide
Peroxynitrite
Single cell gel electrophoresis

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2004.11.052

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title = "Scavenging effects of lotus seed extracts on reactive nitrogen species",

abstract = "The inhibitory effect of lotus (Nelumbo nicifera) seed extracts extracted with water (LSWE), ethyl acetate (LSEAE) and hexane (LSHE) on reactive nitrogen species, induced by DNA damage in macrophage RAW 264.7 cells, was investigated. The results showed that all extracts could inhibit nitric oxide accumulation in LPS-activated RAW 264.7 cells. The extracts in the range of 0.01-0.2 mg/ml showed a dose-dependent inhibitory effect on the accumulation of nitric oxide upon decomposition of sodium nitroprusside (SNP). The potency of inhibitory activity was in the order: LSEAE > LSWE > LSHE. The results of the comet assay indicated that the three extracts could inhibit DNA damage in macrophage RAW 264.7 cells induced by SNP. In addition, the three samples, at 0.2 mg/ml, showed 63%, 59%, and 38% inhibition of DNA damage in macrophage RAW 264.7 cells induced by peroxynitrite, respectively. All extracts tested were found to be potent peroxynitrite scavengers, capable of preventing the nitration of tyrosine. The data obtained suggest that lotus seed extracts might act as chemopreventers through reduction of excess amounts of nitric oxide.",

keywords = "Lotus seed, Macrophage RAW 264.7, Nitric oxide, Peroxynitrite, Single cell gel electrophoresis",

author = "Yen, {Gow Chin} and Duh, {Pin Der} and Su, {Hui Ju} and Yeh, {Chi Tai} and Wu, {Chi Hao}",

note = "Funding Information: This work is part of a research project, 90AS-3.1.3-FD-Z1(13), supported by the Council of Agriculture, Republic of China.",

year = "2006",

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doi = "10.1016/j.foodchem.2004.11.052",

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AU - Yen, Gow Chin

AU - Duh, Pin Der

AU - Su, Hui Ju

AU - Yeh, Chi Tai

AU - Wu, Chi Hao

N1 - Funding Information: This work is part of a research project, 90AS-3.1.3-FD-Z1(13), supported by the Council of Agriculture, Republic of China.

PY - 2006/3

Y1 - 2006/3

N2 - The inhibitory effect of lotus (Nelumbo nicifera) seed extracts extracted with water (LSWE), ethyl acetate (LSEAE) and hexane (LSHE) on reactive nitrogen species, induced by DNA damage in macrophage RAW 264.7 cells, was investigated. The results showed that all extracts could inhibit nitric oxide accumulation in LPS-activated RAW 264.7 cells. The extracts in the range of 0.01-0.2 mg/ml showed a dose-dependent inhibitory effect on the accumulation of nitric oxide upon decomposition of sodium nitroprusside (SNP). The potency of inhibitory activity was in the order: LSEAE > LSWE > LSHE. The results of the comet assay indicated that the three extracts could inhibit DNA damage in macrophage RAW 264.7 cells induced by SNP. In addition, the three samples, at 0.2 mg/ml, showed 63%, 59%, and 38% inhibition of DNA damage in macrophage RAW 264.7 cells induced by peroxynitrite, respectively. All extracts tested were found to be potent peroxynitrite scavengers, capable of preventing the nitration of tyrosine. The data obtained suggest that lotus seed extracts might act as chemopreventers through reduction of excess amounts of nitric oxide.

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KW - Nitric oxide

KW - Peroxynitrite

KW - Single cell gel electrophoresis

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Scavenging effects of lotus seed extracts on reactive nitrogen species

Abstract

Keywords

ASJC Scopus subject areas

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