SARS-CoV regulates immune function-related gene expression in human monocytic cells

Wanchung Hu, Yu Ting Yen, Sher Singh, Chuan Liang Kao, Betty A. Wu-Hsieh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Severe acute respiratory syndrome (SARS) is characterized by acute respiratory distress syndrome (ARDS) and pulmonary fibrosis, and monocytes/macrophages are the key players in the pathogenesis of SARS. In this study, we compared the transcriptional profiles of SARS coronavirus (SARS-CoV)-infected monocytic cells against that infected by coronavirus 229E (CoV-229E). Total RNA was extracted from infected DC-SIGN-transfected monocytes (THP-1-DC-SIGN) at 6 and 24 h after infection, and the gene expression was profiled in oligonucleotide-based microarrays. Analysis of immune-related gene expression profiles showed that at 24 h after SARS-CoV infection: (1) IFN-α/β-inducible and cathepsin/proteasome genes were downregulated; (2) hypoxia/hyperoxia-related genes were upregulated; and (3) TLR/TLR-signaling, cytokine/cytokine receptor-related, chemokine/chemokine receptor-related, lysosome-related, MHC/chaperon-related, and fibrosis-related genes were differentially regulated. These results elucidate that SARS-CoV infection regulates immune-related genes in monocytes/macrophages, which may be important to the pathogenesis of SARS.

Original languageEnglish
Pages (from-to)277-288
Number of pages12
JournalViral Immunology
Volume25
Issue number4
DOIs
Publication statusPublished - 2012 Aug 1

ASJC Scopus subject areas

  • Immunology
  • Molecular Medicine
  • Virology

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