Sampling and profiling caffeine and its metabolites from an eyelid using a watercolor pen based on electrospray ionization/mass spectrometry

Yea Wenn Liou, Kai Yin Chang, Cheng-Huang Lin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A novel and non-invasive sampling method was developed for use in electrospray ionization/mass spectrometry (ESI/MS). The sampling tool, a watercolor pen (brush) that had been rinsed with ethanol, was used to collect analytes from the eyelids of volunteers. Following this, the brush was quickly moved to the front of the mass inlet, where the ethanol as well as the analytes evaporated very fast and escaped from the brush surface. When the analytes make contact with the ESI plume, which arises from the ESI needle tip, they are ionized and then detected by a mass spectrometer. The findings show that this new technique is applicable for the analysis of caffeine and its metabolites in samples obtained from eyelids after the volunteers consumed coffee. The methodology is simple and economical, non-invasive, and is suitable for use in monitoring the levels of caffeine and its metabolites over an extended period of time.

Original languageEnglish
Pages (from-to)51-55
Number of pages5
JournalInternational Journal of Mass Spectrometry
Volume422
DOIs
Publication statusPublished - 2017 Nov 1

Fingerprint

Caffeine
caffeine
pens
Electrospray ionization
metabolites
brushes
Brushes
Metabolites
Mass spectrometry
mass spectroscopy
sampling
Sampling
ionization
Ethanol
ethyl alcohol
coffee
Coffee
Mass spectrometers
needles
Needles

Keywords

  • Caffeine
  • ESI/mass spectrometry
  • Metabolite
  • Non-invasive sampling
  • Watercolor pen

ASJC Scopus subject areas

  • Instrumentation
  • Condensed Matter Physics
  • Spectroscopy
  • Physical and Theoretical Chemistry

Cite this

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abstract = "A novel and non-invasive sampling method was developed for use in electrospray ionization/mass spectrometry (ESI/MS). The sampling tool, a watercolor pen (brush) that had been rinsed with ethanol, was used to collect analytes from the eyelids of volunteers. Following this, the brush was quickly moved to the front of the mass inlet, where the ethanol as well as the analytes evaporated very fast and escaped from the brush surface. When the analytes make contact with the ESI plume, which arises from the ESI needle tip, they are ionized and then detected by a mass spectrometer. The findings show that this new technique is applicable for the analysis of caffeine and its metabolites in samples obtained from eyelids after the volunteers consumed coffee. The methodology is simple and economical, non-invasive, and is suitable for use in monitoring the levels of caffeine and its metabolites over an extended period of time.",
keywords = "Caffeine, ESI/mass spectrometry, Metabolite, Non-invasive sampling, Watercolor pen",
author = "Liou, {Yea Wenn} and Chang, {Kai Yin} and Cheng-Huang Lin",
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AU - Liou, Yea Wenn

AU - Chang, Kai Yin

AU - Lin, Cheng-Huang

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N2 - A novel and non-invasive sampling method was developed for use in electrospray ionization/mass spectrometry (ESI/MS). The sampling tool, a watercolor pen (brush) that had been rinsed with ethanol, was used to collect analytes from the eyelids of volunteers. Following this, the brush was quickly moved to the front of the mass inlet, where the ethanol as well as the analytes evaporated very fast and escaped from the brush surface. When the analytes make contact with the ESI plume, which arises from the ESI needle tip, they are ionized and then detected by a mass spectrometer. The findings show that this new technique is applicable for the analysis of caffeine and its metabolites in samples obtained from eyelids after the volunteers consumed coffee. The methodology is simple and economical, non-invasive, and is suitable for use in monitoring the levels of caffeine and its metabolites over an extended period of time.

AB - A novel and non-invasive sampling method was developed for use in electrospray ionization/mass spectrometry (ESI/MS). The sampling tool, a watercolor pen (brush) that had been rinsed with ethanol, was used to collect analytes from the eyelids of volunteers. Following this, the brush was quickly moved to the front of the mass inlet, where the ethanol as well as the analytes evaporated very fast and escaped from the brush surface. When the analytes make contact with the ESI plume, which arises from the ESI needle tip, they are ionized and then detected by a mass spectrometer. The findings show that this new technique is applicable for the analysis of caffeine and its metabolites in samples obtained from eyelids after the volunteers consumed coffee. The methodology is simple and economical, non-invasive, and is suitable for use in monitoring the levels of caffeine and its metabolites over an extended period of time.

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