Roles of Structure and Structural Dynamics in the Antibody Recognition of the Allergen Proteins: An NMR Study on Blomia tropicalis Major Allergen

Mandar T. Naik, Chi Fon Chang, I. Chun Kuo, Camy C.H. Kung, Fong Cheng Yi, Kaw Yan Chua, Tai Huang Huang

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Blo t 5 is the major allergen from Blomia tropicalis mites and shows strong IgE reactivity with up to 90% of asthmatic and rhinitis patients' sera. The NMR solution structure of Blo t 5 comprises three long α helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. TROSY-NMR experiments were used to study Blo t 5 interaction with the Fab′ of a specific monoclonal antibody, mAb 4A7. The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to undergo conformational exchange in the millisecond to microsecond range. The results presented are critical for the design of a hypoallergenic Blo t 5 for efficacious immunotherapy of allergic diseases.

Original languageEnglish
Pages (from-to)125-136
Number of pages12
JournalStructure
Volume16
Issue number1
DOIs
Publication statusPublished - 2008 Jan 8

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Allergens
Epitopes
Antibodies
Immunoglobulin E
Proteins
Mites
Rhinitis
Immunotherapy
Monoclonal Antibodies
Serum

Keywords

  • MOLIMMUNO
  • PROTEINS

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Roles of Structure and Structural Dynamics in the Antibody Recognition of the Allergen Proteins : An NMR Study on Blomia tropicalis Major Allergen. / Naik, Mandar T.; Chang, Chi Fon; Kuo, I. Chun; Kung, Camy C.H.; Yi, Fong Cheng; Chua, Kaw Yan; Huang, Tai Huang.

In: Structure, Vol. 16, No. 1, 08.01.2008, p. 125-136.

Research output: Contribution to journalArticle

Naik, Mandar T. ; Chang, Chi Fon ; Kuo, I. Chun ; Kung, Camy C.H. ; Yi, Fong Cheng ; Chua, Kaw Yan ; Huang, Tai Huang. / Roles of Structure and Structural Dynamics in the Antibody Recognition of the Allergen Proteins : An NMR Study on Blomia tropicalis Major Allergen. In: Structure. 2008 ; Vol. 16, No. 1. pp. 125-136.
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