Rhcg1 and NHE3b are involved in ammonium-dependent sodium uptake by zebrafish larvae acclimated to low-sodium water

Tin Han Shih, Jiun Lin Horng, Sian Tai Liu, Pung Pung Hwang, Li-Yih Lin

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

To investigate whether Na + uptake by zebrafish is dependent on NH 4 + excretion, a scanning ion-selective electrode technique was applied to measure Na + and NH 4 + gradients at the yolk-sac surface of zebrafish larvae. Low-Na + acclimation induced an inward Na + gradient (uptake), and a combination of low Na + and high NH 4 + induced a larger inward Na + gradient. When measuring the ionic gradients, raising the external NH 4 + level (5 mM) blocked NH 4 + excretion and Na + uptake; in contrast, raising the external Na + level (10 mM) simultaneously enhanced Na + uptake and NH 4 + excretion. The addition of MOPS buffer (5 mM), which is known to block NH 4 + excretion, also suppressed Na + uptake. These results showed that Na + uptake and NH 4 + excretion by larval skin are associated when ambient Na + level is low. Knockdown of Rhcg1 translation with morpholino-oligonucleotides decreased both NH 4 + excretion and Na + uptake by the skin and Na + content of whole larvae. Knockdown of nhe3b translation or inhibitor (5-ethylisopropyl amiloride) treatment also decreased both the NH 4 + excretion and Na + uptake. This study provides loss-offunction evidence for the involvement of Rhcg1 and NHE3b in the ammonium-dependent Na + uptake mechanism in zebrafish larvae subjected to low-Na + water.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume302
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Zebrafish
Ammonium Compounds
Larva
Sodium
Water
Ion-Selective Electrodes
Morpholinos
Yolk Sac
Skin
Acclimatization
Buffers

Keywords

  • Acid-base regulation
  • Na /H exchanger
  • Osmoregulation
  • Rhesus glycoprotein
  • Transporters

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Rhcg1 and NHE3b are involved in ammonium-dependent sodium uptake by zebrafish larvae acclimated to low-sodium water. / Shih, Tin Han; Horng, Jiun Lin; Liu, Sian Tai; Hwang, Pung Pung; Lin, Li-Yih.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 302, No. 1, 01.01.2012.

Research output: Contribution to journalArticle

@article{a31c13067d1b4c2bb3f4211361ca5e7d,
title = "Rhcg1 and NHE3b are involved in ammonium-dependent sodium uptake by zebrafish larvae acclimated to low-sodium water",
abstract = "To investigate whether Na + uptake by zebrafish is dependent on NH 4 + excretion, a scanning ion-selective electrode technique was applied to measure Na + and NH 4 + gradients at the yolk-sac surface of zebrafish larvae. Low-Na + acclimation induced an inward Na + gradient (uptake), and a combination of low Na + and high NH 4 + induced a larger inward Na + gradient. When measuring the ionic gradients, raising the external NH 4 + level (5 mM) blocked NH 4 + excretion and Na + uptake; in contrast, raising the external Na + level (10 mM) simultaneously enhanced Na + uptake and NH 4 + excretion. The addition of MOPS buffer (5 mM), which is known to block NH 4 + excretion, also suppressed Na + uptake. These results showed that Na + uptake and NH 4 + excretion by larval skin are associated when ambient Na + level is low. Knockdown of Rhcg1 translation with morpholino-oligonucleotides decreased both NH 4 + excretion and Na + uptake by the skin and Na + content of whole larvae. Knockdown of nhe3b translation or inhibitor (5-ethylisopropyl amiloride) treatment also decreased both the NH 4 + excretion and Na + uptake. This study provides loss-offunction evidence for the involvement of Rhcg1 and NHE3b in the ammonium-dependent Na + uptake mechanism in zebrafish larvae subjected to low-Na + water.",
keywords = "Acid-base regulation, Na /H exchanger, Osmoregulation, Rhesus glycoprotein, Transporters",
author = "Shih, {Tin Han} and Horng, {Jiun Lin} and Liu, {Sian Tai} and Hwang, {Pung Pung} and Li-Yih Lin",
year = "2012",
month = "1",
day = "1",
doi = "10.1152/ajpregu.00318.2011",
language = "English",
volume = "302",
journal = "American Journal of Physiology - Regulatory Integrative and Comparative Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "1",

}

TY - JOUR

T1 - Rhcg1 and NHE3b are involved in ammonium-dependent sodium uptake by zebrafish larvae acclimated to low-sodium water

AU - Shih, Tin Han

AU - Horng, Jiun Lin

AU - Liu, Sian Tai

AU - Hwang, Pung Pung

AU - Lin, Li-Yih

PY - 2012/1/1

Y1 - 2012/1/1

N2 - To investigate whether Na + uptake by zebrafish is dependent on NH 4 + excretion, a scanning ion-selective electrode technique was applied to measure Na + and NH 4 + gradients at the yolk-sac surface of zebrafish larvae. Low-Na + acclimation induced an inward Na + gradient (uptake), and a combination of low Na + and high NH 4 + induced a larger inward Na + gradient. When measuring the ionic gradients, raising the external NH 4 + level (5 mM) blocked NH 4 + excretion and Na + uptake; in contrast, raising the external Na + level (10 mM) simultaneously enhanced Na + uptake and NH 4 + excretion. The addition of MOPS buffer (5 mM), which is known to block NH 4 + excretion, also suppressed Na + uptake. These results showed that Na + uptake and NH 4 + excretion by larval skin are associated when ambient Na + level is low. Knockdown of Rhcg1 translation with morpholino-oligonucleotides decreased both NH 4 + excretion and Na + uptake by the skin and Na + content of whole larvae. Knockdown of nhe3b translation or inhibitor (5-ethylisopropyl amiloride) treatment also decreased both the NH 4 + excretion and Na + uptake. This study provides loss-offunction evidence for the involvement of Rhcg1 and NHE3b in the ammonium-dependent Na + uptake mechanism in zebrafish larvae subjected to low-Na + water.

AB - To investigate whether Na + uptake by zebrafish is dependent on NH 4 + excretion, a scanning ion-selective electrode technique was applied to measure Na + and NH 4 + gradients at the yolk-sac surface of zebrafish larvae. Low-Na + acclimation induced an inward Na + gradient (uptake), and a combination of low Na + and high NH 4 + induced a larger inward Na + gradient. When measuring the ionic gradients, raising the external NH 4 + level (5 mM) blocked NH 4 + excretion and Na + uptake; in contrast, raising the external Na + level (10 mM) simultaneously enhanced Na + uptake and NH 4 + excretion. The addition of MOPS buffer (5 mM), which is known to block NH 4 + excretion, also suppressed Na + uptake. These results showed that Na + uptake and NH 4 + excretion by larval skin are associated when ambient Na + level is low. Knockdown of Rhcg1 translation with morpholino-oligonucleotides decreased both NH 4 + excretion and Na + uptake by the skin and Na + content of whole larvae. Knockdown of nhe3b translation or inhibitor (5-ethylisopropyl amiloride) treatment also decreased both the NH 4 + excretion and Na + uptake. This study provides loss-offunction evidence for the involvement of Rhcg1 and NHE3b in the ammonium-dependent Na + uptake mechanism in zebrafish larvae subjected to low-Na + water.

KW - Acid-base regulation

KW - Na /H exchanger

KW - Osmoregulation

KW - Rhesus glycoprotein

KW - Transporters

UR - http://www.scopus.com/inward/record.url?scp=84255173195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84255173195&partnerID=8YFLogxK

U2 - 10.1152/ajpregu.00318.2011

DO - 10.1152/ajpregu.00318.2011

M3 - Article

VL - 302

JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

SN - 0363-6119

IS - 1

ER -